Insights into the effect of imidazolium-based ionic liquids on chemical structure and hydrolytic activity of microbial lipase.

This work studied the effect of the cation alkyl chain length of 1-alkyl-n-methylimidazolium chloride ([Cmim]Cl)-based ILs on the activity of Aspergillus niger lipase. First, the lipase activity in the presence of different ILs concentration over time was determined. ILs with shorter cation alkyl side chain length, namely [Cmim]Cl and [Cmim]Cl, promoted an increase of lipase activity; while, [Cmim]Cl, depending on its concentration, maintained or decreased the enzyme activity.

Time-Domain Nuclear Magnetic Resonance (TD-NMR) and Chemometrics for Determination of Fat Content in Commercial Products of Milk Powder.

This study shows the use of time-domain (TD)-NMR transverse relaxation (T2) data and chemometrics in the nondestructive determination of fat content for powdered food samples such as commercial dried milk products. Most proposed NMR spectroscopy methods for measuring fat content correlate free induction decay or echo intensities with the sample's mass. The need for the sample's mass limits the analytical frequency of NMR determination, because weighing the samples is an additional step in this procedure.