Comparison of sperm quality and DNA integrity in mouse sperm exposed to various cooling velocities and osmotic stress.

The first objective was to compare sperm quality following conventional manual sperm freezing (cryovials held 1, 2, 3, and 4 cm, respectively, above liquid nitrogen (LN(2)) for 10 min, resulting in cooling velocities of approximately -14.9, -10.1, -6.

Physiology and experimental medicine: translational research at the Toronto Hospital For Sick Children.

Physiology and Experimental Medicine at The Hospital For Sick Children (Toronto, ON, Canada) is a multidisciplinary program that brings together significant interdisciplinary and interprofessional activity from different study areas such as: lung and cardiovascular physiology; imaging; metabolism and nutrition; pharmacology; and experimental medicine. The program is comprised of researchers, clinicians, and educators who focus on the study of complex, integrated bodily systems. Special emphasis is placed on investigations which bridge in vitro and in vivo approaches, basic science with clinical applications, experimental models with human disease, and human subject research.

Fresh and frozen-thawed sperm quality, nuclear DNA integrity, invitro fertility, embryo development, and live-born offspring of N-ethyl-N-nitrosourea (ENU) mice.

Efficient collection, freezing, reliable archiving of sperm, and re-derivation of mutant mice are essential components for large-scale mutagenesis programs in the mouse. Induced mutations (i.e.

Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse.

Efficient freezing, archiving, and thawing of sperm are essential techniques to support large scale research programs using mouse models of human disease. The purpose of this study was to investigate the effects of variable combinations and concentrations of cryoprotectants on sperm-assessment parameters of frozen-thawed mouse sperm in order to optimize cryopreservation protocols. Sperm was frozen using combinations of 3% skim milk + 0.