Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity.

Many bacterial species cannot be cultured in the laboratory using standard methods, posing a significant barrier to studying the majority of microbial diversity on earth. Novel approaches are required to culture these uncultured bacteria so that investigators can effectively study their physiology and lifestyle using the powerful tools available in the laboratory. The Candidate Phyla Radiation (CPR) is one of the largest groups of uncultivated bacteria, comprising ~15% of the living diversity on earth.

A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study.

Background: Faecal shedding of SARS-CoV-2 has raised concerns about transmission through faecal microbiota transplantation procedures. Validation parameters of authorised tests for SARS-CoV-2 RNA detection in respiratory samples are described in product labelling, whereas the published methods for SARS-CoV-2 detection from faecal samples have not permitted a robust description of the assay parameters. We aimed to develop and validate a test specifically for detection of SARS-CoV-2 in human stool.

Isolation and cultivation of candidate phyla radiation (TM7) bacteria in coculture with bacterial hosts.

Background: The vast majority of bacteria on earth have not yet been cultivated. There are many bacterial phyla with no cultivated examples including most members of the Candidate Phylum Radiation with the exception of human oral isolates from the phylum Saccharibacteria.

Aims: The aims of this research were to develop reproducible methods and validate approaches for the cultivation of human oral Saccharibacteria and to identify the conceptual pitfalls that delayed isolation of these bacteria for 20 years after their discovery.

Complete Genome Sequence of Strain PM004, a Novel Cultured Member of the Human Oral Microbiome from the Candidate Phylum (TM7).

Strain PM004 is a cultured representative of human microbial taxon 955, a bacterium from the phylum It is an obligate parasite with a genome of <0.9 Mb and can be grown in coculture with its host, The complete genome sequence is presented here.

Complete Genome Sequence of Strain AC001, a Novel Cultured Member of the Human Oral Microbiome from the Candidate Phylum (TM7).

Strain AC001 is a cultured representative of human microbial taxon 488, a bacterium from the candidate phylum It is an obligate parasite with a genome of <0.9 Mb and grows in coculture with its host, The complete genome sequence is presented here.