Publications by authors named "Pallaprolu Nikhil"
Affinity-based protein depletion and TiO enrichment methods play a crucial role in detection of low-abundant proteins and phosphopeptides enrichment, respectively. Here, we assessed the effectiveness of HSA/IgG (HU2) and Human 7 (HU7) depletion methods and their impact on phosphopeptides coverage through comparative proteome analysis, utilizing in-solution digestion and nano-LC-Orbitrap mass spectrometry (MS). Our results demonstrated that both HU2 and HU7 affinity depletion significantly decreased high-abundant proteins by 1.
View Article and Find Full Text PDFThrough a comprehensive molecular docking study, a unique series of naphthoquinones clubbed azetidinone scaffolds was arrived with promising binding affinity to Mycobacterial Cytbc1 complex, a drug target chosen to kill multi-drug resistant Mycobacterium tuberculosis (MDR-Mtb). Five compounds from series-2, 2a, 2c, 2g, 2h, and 2j, showcased significant in vitro anti-tubercular activities against Mtb HRv and MDR clinical isolates. Further, synergistic studies of these compounds in combination with INH and RIF revealed a potent bactericidal effect of compound 2a at concentration of 0.
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- N-Nitroso dimethylamine (NDMA) is a harmful impurity found in ranitidine products, with the potential to form during storage due to the drug's chemical structure, prompting the FDA to recommend antioxidants as a solution.
- A sensitive liquid chromatography/high-resolution mass spectrometry (LC-HRMS) method was developed for detecting NDMA, achieving high sensitivity and precise quantification of NDMA levels in ranitidine samples.
- The study confirmed that antioxidants effectively reduce NDMA formation during nitrosation of ranitidine, with ascorbic acid being the most effective, significantly lowering NDMA levels by nearly 97%.
Article Synopsis
- The study found that caprylic acid (C8), a medium-chain fatty acid, is highly effective against Leishmania donovani, the cause of visceral leishmaniasis.
- Researchers identified ergosterol depletion as the main cause of cell death induced by caprylic acid and its analogs, targeting the enzyme mevalonate kinase (MevK) in the ergosterol synthesis pathway.
- This research provides new insights into a natural compound's mechanism against a pathogen, highlighting the potential for alternative treatments in the context of drug resistance.