[Effect of iodine and cobalt salts on content of biologically active substances in spirulina biomass].

Production technology of iodinized spirulina biomass at vertical panel airlifted photobioreactor has been developed. The influence of different concentrations of iodine ions at certain combinations with cobalt ions on iodine-accumulative properties of spirulina has been studied. It has been shown that it is possible to obtain spirulina biomass containing certain amount of organically bound iodine by varying cobalt and iodine ions combinations and by staged addition of ions to incubation medium.

Adaptor protein Ruk1 forms protein-protein complexes with endonuclease activity in HEK293 cells.

The structural and functional organization of the adaptor protein Ruk(1) is characterized by the presence of three SH3-domains at the N-terminus followed by Pro- and Ser-rich sequences and a C-terminal coiled-coil region. Multiple modules in the Ruk(1) structure involved in protein-protein interactions can provide for formation of ligand clusters with varied properties and subcellular location. To study the nature and biological role of such complexes, the recombinant protein Ruk(1) with a Glu-epitope at the C-terminus (Ruk(1) Glu-tagged) was purified from transfected HEK293 cells by affinity chromatography on protein G-Sepharose with covalently conjugated anti-Glu-tag antibodies.

[Effect of tocopherol and tocopherol quinone complexes with proteins on activity of leukotriene B4 lipoxygenase].

While estimating the effect of tocopherol, tocopherylquinone and their complexes with the tocopherol-binding proteins from the rat liver cytosole on arachidonate 5-lipoxygenase from peritoneal-lymphocytes and soybean linoleate-5-lipoxygenase alpha-tocopherol and especially its complex with tocopherol-binding protein was defined to inhibit the activity of both vegetative- and animal nature-lipoxygenase.

[Effect of the complexes tocopherol-protein-acceptors and tocopherylquinone-protein-acceptors on the activity cytosol phospholipase A2 in rat liver].

A new method of tocopherol and tocopherylquinone binding with rat liver cytosol proteins-acceptors. Complexes tocopherol-proteins-acceptors and tocopheryl-quinone-proteins acceptors have been studied for their effect on phospholipase A2 activity. It is established that the both complexes are the inhibitors of phospholipase A2, under these conditions tocopherylquinone complex with proteins-acceptors more intensively decreases Km of the enzyme than the complex tocopherol-proteins-acceptors.

[In vitro incorporation of [14CH3]methionine into liver phosphatidylcholine in rats with vitamin E deficiency].

The content of phosphatidyl choline, a terminal product of phosphatidyl ethanolamine methylation as well as in intensity in vitro incorporation of [14CH3]methionine into phosphatidyl choline of rat liver with E-hypovitaminosis are found to considerably decrease as compared to the norm. In this case the total content of phospholipids is practically the same.

[The role of vitamin E in the biosynthesis of the isoprenoid region of the ubiquinone molecule in the rat liver].

A decrease in the CoA and acetyl-CoA amount in the rat liver tissue by 34.8 and 29.4%, respectively, as well as inhibition of the biosynthesis rate of mevalonic acid from [I-14C] acetyl-CoA in the postmitochondrial liver fraction by 17.

[Effect of alpha-tocopherol on relations between RNA and ubiquinone biosynthesis in rat liver].

The animals were kept on the low-protein diet deprived of vitamin E. Addition of the latter to the diet fed to a noticeable increase in the intensity of the biosynthesis of total and nuclear RNA of the rat liver. alpha-Tocopherol had a clearly marked stimulant effect on the synthesis of RNA and ubiquinone as shown by in vitro experiments under 30-minute preincubation of the liver from rats with E-hypovitaminosis.

[Alpha-tocopherol and actinomycin D regulation of ubiquinone and protein biosynthesis in rat liver in E-hypovitaminosis].

In vitro experiments with the liver preparations from rats with E-hypovitaminosis revealed a more intensive incorporation of [14C]phenylalanine into ubiquinone induced by alpha-tocopherol. Actinomycin D inhibiting the DNA-dependent RNA synthesis decreases the incorporation intensity. A 30 min preincubation of the liver preparations with thesis.

[Effect of vitamin E an actinomycin D on protein and RNA synthesis in rat liver].

In vitro experiments showed that RNA synthesis intensity in the rat liver with E-hypovitaminosis decreases considerably while the level of the labelled precursors incorporation into protein does not differ from the norm. Under conditions of E-hypovitaminosis the inhibitory effect of actinomycin D on the RNA synthesis is pronounced more clearly as compared to the norm. In the case of the E-hypovitaminous rate liver chyme preincubation with alpha-tocopherol there is no inhibitory effect of actinomycin D on the protein biosynthesis.