Publications by authors named "Pakhomov Oleksandr"
The study is devoted to the effect of lowered resuscitation temperature (26 °C) on cryopreserved porcine adrenal glands functional activity in vitro and in vivo under xenotransplantation. The adrenals were collected from newborn pigs, cryopreserved with 5 % DMSO at a rate of 1 °C/min, resuscitated at 26 or 37 °C for 48 h (5 % CO, DMEM), embedded into small intestinal submucosa, and transplanted to bilaterally adrenalectomized rats. It has been shown that the glands resuscitated at 26 °C have suppressed free-radical processes and can produce cortisol and aldosterone in vitro, and may lead to elevated blood levels of these hormones.
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- The study focuses on developing an affordable microscopy system using Arduino for cryobiology research.
- It investigates the cryoprotection and cryodamage mechanisms in testis interstitial cells using various cryoprotective agents, particularly analyzing the effects of a specific serum-free medium.
- Results indicate that this new medium, containing lower concentrations of cryoprotectants, reduces ice formation and enhances cell survival post-cryopreservation, suggesting potential for less toxic solutions in lab and clinical settings.
The development of serum-/xeno-free media may help avoid the drawbacks of using serum and its components, such as probable contamination, instability of composition, or difficulty in sterilization. The objectives of this research were to investigate the use of combinations of a permeating cryoprotective agent (MeSO) and non-permeating polymers (polyvinyl alcohol, polyvinylpyrrolidone, polyethylene glycol, hydroxyethyl starch, dextran) for cryopreservation of interstitial cells (ICs) of rat testis, and to propose the mechanism of cryoprotection of such compositions. In the course of this study, the best combination was 100 mg/ml dextran (M.
View Article and Find Full Text PDFObjective: The aim: of the work was to study the antiviral activity of the metabolites of the probiotic strain Lactobacillus rhamnosus GG (LGG or ATCC 53103) regarding clinical strains of enteroviruses (Coxsackie B-5, ECNO21) isolated from the feces of intestinal infections.
Patients And Methods: Materials and methods: The object of the study was substrate-dependent cell cultures of HeLa, Vero, Hep-2 lines. The titer of the virus was determined by the presence of a clear cytopathic action (CPA) in the monolayer infected cells of the virus.
Article Synopsis
- Transplanting hormone-producing interstitial cell suspension (ICS) is being studied as a treatment for endocrine dysfunction in rats, particularly focusing on its effects on sexual behavior following orchidectomy (OE).
- The study involved two populations of adult rats with varying prior sexual experiences, split into control groups and orchidectomized groups with one receiving ICS treatment.
- Results showed that while OE initially decreased sexual function, ICS transplantation helped restore sexual activity over four weeks by increasing free testosterone levels and maintaining male sexual behavior.