Limosilactobacillus reuteriKUB-AC5 andLactobacillus johnsoniiKUNN19-2 were spray-dried using whey protein isolate, sucrose, and whey protein isolate combined with sucrose at 130 °C and 150 °C. Differences in membrane fatty acid compositions of probiotics led to differences in cell membrane fluidity, cell injury, and cell survival during spray drying. Combination of whey protein isolate and sucrose improved survival of L.
View Article and Find Full Text PDFEach year, sugarcane bagasse, a low-priced by-product of the sugar industry, is generated in large quantities. The aim of this study was to optimize the alkaline hydrolysis condition for the extraction of lignin from sugarcane bagasse using response surface methodology combined with Box-Behnken design, and to evaluate functional properties of lignin extracts for cosmetic applications. Three process parameters were varied (NaOH solution concentrations (3-7% w/v), temperatures (115-135 °C), and times (30-60 min)).
View Article and Find Full Text PDFDespite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles. This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation. After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.
View Article and Find Full Text PDFLipid peroxidation induced by oxidative stress plays an important role in reducing probiotic survival during spray drying. In this study, the probiotic Lactobacillus reuteri KUB-AC5 was co-encapsulated with holy basil essential oil, HBEO (0, 6, and 12mg/ml), by spray drying using skim milk at inlet air temperatures of 130, 140, and 150°C. The microcapsule properties in terms of morphology, size, moisture content, water activity, surface oil, microencapsulation efficiency, antioxidant activity, lipid peroxidation, and cell survival were determined.
View Article and Find Full Text PDFHoly basil essential oils (HBEO) can be used in many food applications due to antioxidant and antimicrobial attributes, but they are susceptible to degradation upon storage. Therefore, a protective system is required to extend their shelf life. HBEO was microencapsulated by coacervation using gelatin and the microcapsules were subsequently coated with stearic acid (1%, 2%, and 3%) in carboxymethyl cellulose emulsions.
View Article and Find Full Text PDFHoly basil essential oil (HBEO) can be applied as a feed additive; however, its benefits depend on the available amount in the gastrointestinal tract. In this study, the physicochemical properties, including the release properties of three different microcapsules, HBEO-loaded gelatin microcapsules (UC), UC coated with aluminum carboxymethyl cellulose (CC), and UC coated with aluminum carboxymethyl cellulose-beeswax composite (CB), were compared. The encapsulation efficiency, HBEO content, and 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity for the microcapsules were 95.
View Article and Find Full Text PDFTo protect holy basil essential oil (HBEO) from volatilisation and oxidation, microencapsulation by simple coacervation of gelatin was developed. An optimal encapsulating condition obtained from response surface methodology (RSM) was a gelatin concentration of 11.75% (w/v) and an HBEO amount of 31ml, which provided the greatest yield, oil content, and encapsulation efficiency of 98.
View Article and Find Full Text PDFIndustrial firms that kill and process chickens generate wastewater that contains fat, oil, and grease (FOG). The FOGs are located in the fatty waste that is collected by floatation in grease traps. Chemical and physical characterisation of FOGs would provide useful information that would help in the development of methods designed to decrease the extent of pollution caused by disposal of the waste and to utilise commercially some of its lipid constituents.
View Article and Find Full Text PDFThis research aimed to enhance the survival of Lactobacillus reuteri KUB-AC5 from heat conditioning by using microencapsulation with aluminum carboxymethyl cellulose-rice bran (AlCMC-RB) composites of different weight ratios of 1:0, 1:1, and 1:1.5. The cell/polymer suspension was crosslinked with aluminum chloride at different agitation speeds of 1200, 1500, and 2100 rpm.
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