[Evaluation of interactions between strains of S. aureus isolated from different clinical specimens with peripheral blood phagocytic cells].

The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal elutriation were incubated with suspensions of opsonized bacteria.

[Evaluation of the interactions between strains of S. aureus and peripheral blood phagocytic cells].

The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria.

[Properties of Pseudomonas aeruginosa strains. Adherence and proteolytic activity].

The material consisted of 141 strains of Pseudomonas aeruginosa isolated from various sources and derived from children treatment in the Institute of Pediatrics. Adherence properties of these strains were tested by application of an own micromethod with application of 96 well titration plates. Proteolytic activity was investigated by means of gel radial diffusion with application of caseine and elastin as substrates.

[Chemiluminescence of granulocytes in response to Pseudomonas strains isolated from various materials].

The study was aimed at comparison of chemiluminescence of granulocytes during their incubation with suspensions of Pseudomonas strains isolated from various sources. The study was performed on 136 strains isolated from clinical material. The results were correlated with resistance of these bacteria to bactericidal activity of serum and their ability to produce proteolytic enzymes.

Characteristics of clinical isolates of Pseudomonas aeruginosa. I. Resistance to serum bactericidal effect and production of proteolytic enzymes.

136 strains of Pseudomonas aeruginosa were tested for human serum bactericidal effect. Hundred (73%) of clinical isolates were resistant and 36 (27%) were effectively killed by human (undiluted or even 1000 times diluted) serum. Fifty six out of 136 (41.

Detection of inhibitory inactive fraction of alpha-1-antitrypsin in human serum.

Pseudomonas aeruginosa elastase is not inhibited by human serum alpha-1-antitrypsin, it damages inhibitory activity of alpha-1-antitrypsin activity instead. A simple method, based on electrophoresis in urea containing polyacrylamide gel, is described for the separation of active part of the inhibitor from that inactivated by the bacterial enzyme.

Interaction of elastase from Pseudomonas aeruginosa with polymorphonuclear leukocytes and serum.

Interaction of elastase from Pseudomonas aeruginosa 700/75 with polymorphonuclear leukocytes and serum was tested. No difference in phagocytosis by elastase treated PMN and control PMN was noted. Bactericidal activity of serum against P.

Functional changes of macrophages from polyoma tumor-bearing mice.

CBA mice were inoculated with different doses of polyoma tumor cells. The PHA response of spleen cells from tumor-bearers was decreased but the spontaneous incorporation of 3H-leucine was increased. PHA-induced cytotoxicity of spleen cells was increased only in tumor-bearers.

Purification and properties of L-asparaginase EC-2 from Escherichia coli 055:B5.

1. L-Asparaginase has been isolated from aerobically grown Escherichia coli 055:B5 and purified about 140-fold in a three-step procedure involving acidification to pH 4.5, ammonium sulphate fractionation and column chromatography on DEAE-Sephadex A-50.

[Immunological aspects in the L-asparaginase treatment of children with lymphoproliferative diseases].

A group of 20 children, including 14 with acute lymphoblastic leukemia and 6 with lymphosarcoma, was studied. 24 cures of l-asparaginase therapy were carried out. The increase of serum immunoglobulin (IgG, IgA, IgM) levels was found in children treated with smaller (from 300 to 500 I.