Publications by authors named "Padilla J"

The traditional method of CAR T cell production involves lengthy ex-vivo culture times which can result in the reduction of crucial naïve T cell subsets. Moreover, traditional CAR T cell therapy manufacturing processes can prolong time-to-patient, potentially delaying patient treatment, and contribute to disease progression. In this study, we describe an innovative and semi-automated 24-hour CAR T manufacturing process that yields a higher percentage of naïve/stem-cell like T cells which showed high cytotoxic activity and cytokine release in vitro.

View Article and Find Full Text PDF
Article Synopsis
  • Endothelial dysfunction in type 2 diabetes (T2D) may be caused by stiffening of cell structures, possibly linked to polymerization of filamentous actin (F-actin) and its regulation by the protein cofilin.
  • Oxidative stress in endothelial cells from T2D patients and diabetic mice leads to increased F-actin and cell stiffness, with reactive oxygen species promoting F-actin formation and inactivating cofilin.
  • Genetic silencing or inhibiting LIM kinase 1, which inactivates cofilin, can decrease F-actin levels and reduce cell stiffness, suggesting a novel mechanism for endothelial stiffening in T2D.
View Article and Find Full Text PDF

Background: Protein-coding genes have been considered the functional part of the genome, although they represent only 2% of the genome. In contrast, more than 90% of the genome produces non-coding RNA (ncRNA), including antisense (AS) genes, a type of long non-coding genes (encoding transcripts > 200 nucleotides) located on the opposite strand of coding genes. Therefore, antisense RNA (asRNA) can be complementary to the counterpart sense RNA, supporting a regulatory role with potential pathogenic consequences, as their deregulation has been associated with cardiovascular disease, cancer, and diabetes.

View Article and Find Full Text PDF

Background: Antimicrobial-resistant bacteria represent a serious threat to public health. Among these bacteria, is of high priority because of its morbidity levels and its ability to induce different types of cancer.

Aim: This study aimed to identify strains encoding genes linked to the promotion of precancerous lesions and to isolate a bacteriophage to evaluate its preclinical potential against these bacteria.

View Article and Find Full Text PDF

Insulin has important vasodilatory effects in the peripheral circulation, but less is known about insulin's role in cerebrovascular control. Herein, we hypothesized both systemic (intravenous) and local (intranasal) insulin administration would increase indices of cerebral blood flow and reduce cerebrovascular compliance (Ci) in young adults. Participants were assigned to one of four separate protocols.

View Article and Find Full Text PDF

Unlabelled: During myognesis, myonuclei are actively moved during embryogenesis, and their spacing is maintained through an anchoring mechanism in the fully differentiated myofiber. While we have identified microtubule associated proteins, motors, and nuclear envelope proteins that regulate myonuclear spacing, the developmental time during which each gene functions has not been tested. Here we have identified a as required only for the maintenance of myonuclear spacing.

View Article and Find Full Text PDF
Article Synopsis
  • The left ventricular summit (LVS) is a key area where epicardial arrhythmias frequently occur, making it a significant focus for medical professionals.
  • Ablating arrhythmias at the LVS is particularly challenging due to its complicated anatomical structure and proximity to other vital heart areas.
  • This review provides an in-depth analysis of the LVS anatomy and offers a thorough overview of the techniques used for mapping and ablation of LVS-related arrhythmias.
View Article and Find Full Text PDF

Degradation of the endothelial glycocalyx in type 2 diabetes (T2D) is thought to contribute to impaired shear stress mechanotransduction, leading to endothelial dysfunction and the development of cardiovascular disease. Herein, we tested the hypothesis that restoration of the endothelial glycocalyx with dietary supplementation of glycocalyx precursors (DSGPs, containing glucosamine sulfate, fucoidan, superoxide dismutase, and high-molecular weight hyaluronan) improves endothelial function and other indices of vascular function in T2D. First, in db/db mice, we showed that treatment with DSGP (100 mg/kg/day) for 4 wk restored endothelial glycocalyx length, as assessed via atomic force microscopy in aortic explants.

View Article and Find Full Text PDF

Objective: When patients with non-traumatic lower extremity conditions express concerns regarding symptoms of the contralateral lower extremity they may be communicating fear of painful movement. Among people seeking specialty care for unilateral non-traumatic lower extremity conditions, we studied the relationship between magnitude of incapability and pain intensity in the contralateral and ipsilateral limbs and thoughts and feelings about sensations.

Methods: Patients seeing a musculoskeletal specialist for any unilateral non-traumatic lower extremity condition were enrolled.

View Article and Find Full Text PDF

and are frequent causes of healthcare-associated infections. Antimicrobial-resistant enterococci pose a serious public health threat, particularly vancomycin-resistant enterococci (VRE), for which treatment options are limited. The Centers for Disease Control and Prevention's Division of Healthcare Quality Promotion Sentinel Surveillance system conducted surveillance from 2018 to 2019 to evaluate antimicrobial susceptibility profiles and molecular epidemiology of 205 and 180 clinical isolates collected from nine geographically diverse sites in the United States.

View Article and Find Full Text PDF

Introduction: Lymphocele is a common complication post-kidney transplantation, influenced by various factors including surgical technique, graft vessel count, operator experience, body mass index, ischemia time, and immunotherapy regimens.

Project Aims: The purpose of this study was to evaluate lymphocele risk factors, particularly focusing on the role of end-stage kidney disease.

Design: A retrospective study was conducted on renal transplant recipients from a single center (March 2020 to December 2022).

View Article and Find Full Text PDF

The plant pathogenic bacterium Burkholderia glumae causes bacterial panicle blight (BPB) in rice-growing areas worldwide. It has been widely accepted that an acyl-homoserine lactone (AHL)-type quorum sensing (QS) system encoded by tofI and tofR genes (TofIR QS) is a key regulatory mechanism underlying the bacterial pathogenesis of B. glumae.

View Article and Find Full Text PDF

A bimetallic FeCu/NC core-shell catalyst, consisting in nanoparticles where zero-valent Fe and Cu atoms, slightly oxidized on their surface, are encapsulated by carbon has been successfully prepared by modifying the synthesis route of MIL(Fe)-88B. FeCu/NC possessed well-balanced textural and electrochemical properties. According to voltammetric responses, in-situ Fe(III) reduction to Fe(II) by low-valent Cu was feasible, whereas the high double-layer capacitance confirmed the presence of a great number of electroactive sites that was essential for continuous HO activation to OH via Fenton's reaction.

View Article and Find Full Text PDF

Conventional electro-Fenton (EF) process at acidic pH ∼3 is recognized as a highly effective strategy to degrade organic pollutants; however, homogeneous metal catalysts cannot be employed in more alkaline media. To overcome this limitation, pyrolytic derivatives from metal-organic frameworks (MOFs) have emerged as promising heterogeneous catalysts. Cu-based MOFs were prepared using trimesic acid as the organic ligand and different pyrolysis conditions, yielding a set of nano-Cu/C catalysts that were analyzed by conventional methods.

View Article and Find Full Text PDF

University students often experience psychological strains such as academic stress, particularly as they approach the transition into the workforce. This stress may have been heightened for nursing students who completed internships during the COVID-19 pandemic. This study aims to analyze the impact of the COVID-19 pandemic on the perceived stress levels of undergraduate nursing students.

View Article and Find Full Text PDF

Background: Retinopathy of prematurity (ROP) is a leading cause of avoidable blindness in children, particularly in Latin America, where hyperoxia is a significant risk factor. This study evaluated resource availability and use for administering and monitoring supplemental oxygen in Mexico.

Methods: In 2011, an observational study in which 32 government neonatal intensive care units (NICUs) across Mexico were visited.

View Article and Find Full Text PDF

The lining of blood vessels is constantly exposed to mechanical forces exerted by blood flow against the endothelium. Endothelial cells detect these tangential forces (i.e.

View Article and Find Full Text PDF
Article Synopsis
  • A 40-year-old male from Vereda Buenos Aires presented to the emergency department with symptoms including fever, headache, and dysuria, initially diagnosed with a urinary tract infection.
  • After five days, he was confirmed to have acute phase Chagas disease due to a positive result for Trypanosoma cruzi.
  • The patient received treatment with nifurtimox and benznidazole, and health authorities conducted investigations in the community, identifying five other symptomatic individuals, but further tests for Chagas were negative, leading to health education initiatives to prevent disease spread.
View Article and Find Full Text PDF

Coffee berry borer (CBB) Hypothenemus hampei is a major biotic threat to coffee production worldwide. Studies have reported negative effects on CBB by oil-based formulations of neem (Azadirachta indica), but little information is available for other neem-extract formulations. This study evaluated CBB preference and performance in arabica coffee fruits and artificial diet treated with a neem-extract formulation (Openeem Plus®) in the field and laboratory conditions.

View Article and Find Full Text PDF

Background: Routine histopathologic examination of orthopaedic surgical specimens is a standard practice at many institutions. Previous studies have demonstrated that this practice seldom altered patient management for several orthopaedic procedures. As a result, the value of such practices has come into question.

View Article and Find Full Text PDF

Chronic obstructive pulmonary disease (COPD) is regarded as an accelerated-age disease in which chronic inflammation, maladaptive immune responses, and senescence cell burden coexist. Accordingly, cellular senescence has emerged as a potential mechanism involved in COPD pathophysiology. In this study, 25 stable patients with COPD underwent a daily physical activity promotion program for 6 mo.

View Article and Find Full Text PDF

Background: Chronic stress causes harmful physiological responses that yield increased inflammation and subsequent health conditions. Stress is an important measure among minoritized populations who face social situations that predispose risk to developing mental health problems. Hair and fingernail cortisol have been studied as retrospective measures of chronic stress and to demonstrate biological response to social situations.

View Article and Find Full Text PDF

Sibling relational aggression is an understudied social and family process that is of developmental significance in adolescence, a period of interpersonal relationship development. This study examined developmental change in sibling relational aggression across adolescence and used multilevel actor-partner independence models to test its longitudinal associations with mother-adolescent, father-adolescent, and sibling relationship qualities. Participants were 196 adolescent (firstborn-secondborn) sibling pairs from predominantly White families who averaged 16.

View Article and Find Full Text PDF

Hair and nail cortisol is increasingly studied as a physiologic proxy for chronic stress response. Glucocorticoid use is an expected confounder for cortisol measurement, yet there remains little evidence of whether external cortisol use should be subject to exclusion in study subjects. In a group of 209 youth (15-22 year-olds), we analyzed hair and fingernail cortisol concentrations.

View Article and Find Full Text PDF
Article Synopsis
  • - The study investigates how using pedogenic ironstone and untreated red mud can help reduce phosphorus (P) loss in water runoff, which causes eutrophication in rivers and lakes.
  • - Both materials showed similar P absorption capabilities, but red mud's absorption improved significantly when the pH dropped, making it more effective at retaining phosphorus compared to ironstone when mixed with soil.
  • - While ironstone didn't affect soil drainage, adding even a small amount of red mud did; however, red mud also boosted the growth and phosphorus uptake of common bermudagrass, suggesting its potential use in permeable reactive barriers (PRB) for environmental management.
View Article and Find Full Text PDF