A New Species of Hemicotelles (Hymenoptera: Colletidae) from Peru.

We diagnose, describe and illustrate Hemicotelles misti Anaya, Huanca and Packer n. sp., from the slopes of the Misti Volcano in Arequipa Province, Peru.

A survey of keys for the identification of newly described insect genera: recommendations for authors, reviewers, editors, and publishers.

Large numbers of new taxa are described annually and while there is a great need to make them identifiable, there seems little consistency in how this might be facilitated. 427 papers published in 2021 and 2022 were surveyed, which described 587 new insect genera. Only 136 of these papers included keys, and these allowed the identification of 233 of the new genera (31.

A new ground level neutron monitor for space weather assessment.

We report on a new ground-level neutron monitor design for studying cosmic rays and fluxes of solar energetic particles at the Earth's surface. The first-of-its-kind instrument, named the NM-2023 after the year it was standardised and following convention, will be installed at a United Kingdom Meteorological Office observatory (expected completion mid 2024) and will reintroduce such monitoring in the UK for the first time since ca. 1984.

Three New Species of Microsphecodes s.str. (Hymenoptera: Halictidae) with an updated key to species of the subgenus.

We diagnose, describe and illustrate three new species of Microsphecodes Eickwort and Stage, all from the nominate subgenus: M. peckorum Graham and Packer n. sp.

The evolutionary history of bees in time and space.

Bees are the most significant pollinators of flowering plants. This partnership began ca. 120 million years ago, but the uncertainty of how and when bees spread across the planet has greatly obscured investigations of this key mutualism.

Reprint of: Photoperoxidation in Isolated Chloroplasts I. Kinetics and Stoichiometry of Fatty Acid Peroxidation.

A photo-induced cyclic peroxidation in isolated chloroplasts is described. In an osmotic buffered medium, chloroplasts upon illumination produce malondialdehyde (MDA)-a decomposition product of tri-unsaturated fatty acid hydroperoxides-bleach endogenous chlorophyll, and consume oxygen. These processes show (a) no reaction in the absence of illumination; (b) an initial lag phase upon illumination of 10-20 minutes duration; (c) a linear phase in which the rate is proportional to the square root of the light intensity; (d) cessation of reaction occurring within 3 minutes after illumination ceases; and (e) a termination phase after several hours of illumination.

Patient-derived xenograft models capture genomic heterogeneity in endometrial cancer.

Background: Endometrial cancer (EC) is a major gynecological cancer with increasing incidence. It comprises four molecular subtypes with differing etiology, prognoses, and responses to chemotherapy. In the future, clinical trials testing new single agents or combination therapies will be targeted to the molecular subtype most likely to respond.

Convolutional Neural Networks for Challenges in Automated Nuclide Identification.

Improvements in Radio-Isotope IDentification (RIID) algorithms have seen a resurgence in interest with the increased accessibility of machine learning models. Convolutional Neural Network (CNN)-based models have been developed to identify arbitrary mixtures of unstable nuclides from gamma spectra. In service of this, methods for the simulation and pre-processing of training data were also developed.

The evolutionary history of the cellophane bee genus Colletes Latreille (Hymenoptera: Colletidae): Molecular phylogeny, biogeography and implications for a global infrageneric classification.

Colletes Latreille (Hymenoptera: Colletidae) is a diverse genus with 518 valid species distributed in all biogeographic realms, except Australasia and Antarctica. Here we provide a comprehensive dated phylogeny for Colletes based on Bayesian and maximum likelihood-based analyses of DNA sequence data of six loci: 28S rDNA, cytochrome c oxidase subunit 1, elongation factor-1α copy F2, long-wavelength rhodopsin, RNA polymerase II and wingless. In total, our multilocus matrix consists of 4824 aligned base pairs for 143 species, including 112 Colletes species plus 31 outgroups (one stenotritid and a diverse array of colletids representing all subfamilies).

Fifteen new species of Liphanthus Reed (Hymenoptera: Andrenidae) with two submarginal cells.

Hitherto, the panurgine genus Liphanthus Reed 1894 has been thought to have only a single species with two, as opposed to three, submarginal cells. Here we describe an additional fifteen species with two submarginal cells. These new species are: L.

Phylogeny and biogeography of the cleptoparasitic bee genus Epeolus (Hymenoptera: Apidae) and cophylogenetic analysis with its host bee genus Colletes (Hymenoptera: Colletidae).

The bee genus Epeolus Latreille (Hymenoptera: Apidae) consists of 109 species, which are known to be exclusively cleptoparasites of polyester (or cellophane) bees of the genus Colletes Latreille (Hymenoptera: Colletidae). Both genera have a nearly cosmopolitan distribution and are represented on all continents except Antarctica and Australia. We present the most comprehensive phylogeny for Epeolus to date, based on combined molecular and morphological data.

Fifteen new species of Chilicola (Oroediscelis) (Hymenoptera: Colletidae: Xeromelissinae) with illustrated keys to the males and females of the subgenus.

Fifteen new species of Chilicola subgenus Oroediscelis are described and illustrated. The following 11 species are from Argentina recording a remarkable diversity and the first records of the subgenus in the country: Chilicola capillitas Packer and Dumesh, new species, C. abrebotellas, Packer and Dumesh, new species; C.

Bcl-2 inhibitors enhance FGFR inhibitor-induced mitochondrial-dependent cell death in FGFR2-mutant endometrial cancer.

Endometrial cancer is the most commonly diagnosed gynaecological malignancy. Unfortunately, 15-20% of women demonstrate persistent or recurrent tumours that are refractory to current chemotherapies. We previously identified activating mutations in fibroblast growth factor receptor 2 (FGFR2) in 12% (stage I/II) to 17% (stage III/IV) endometrioid ECs and found that these mutations are associated with shorter progression-free and cancer-specific survival.

A new species of Lepidotrigona (Hymenoptera: Apidae) from Thailand with the description of males of L. flavibasis and L. doipaensis and comments on asymmetrical genitalia in bees.

We describe Lepidotrigona satun Attasopa and Bänziger new species from southern Thailand based upon associated males and females (workers). The new species is a member of the L. ventralis species group, which is otherwise represented in Thailand only by L.

OVERVIEW OF NEUTRON MEASUREMENTS IN JET FUSION DEVICE.

The design and operation of ITER experimental fusion reactor requires the development of neutron measurement techniques and numerical tools to derive the fusion power and the radiation field in the device and in the surrounding areas. Nuclear analyses provide essential input to the conceptual design, optimisation, engineering and safety case in ITER and power plant studies. The required radiation transport calculations are extremely challenging because of the large physical extent of the reactor plant, the complexity of the geometry, and the combination of deep penetration and streaming paths.

Queens and Workers Contribute Differently to Adaptive Evolution in Bumble Bees and Honey Bees.

Eusociality represents a major transition in evolution and is typified by cooperative brood care and reproductive division of labor between generations. In bees, this division of labor allows queens and workers to phenotypically specialize. Worker traits associated with helping are thought to be crucial to the fitness of a eusocial lineage, and recent studies of honey bees (genus Apis) have found that adaptively evolving genes often have worker-biased expression patterns.

A generic approach for simultaneous measurements of total antibody and cleavable antibody-conjugated drug by LC/MS/MS.

The current industry practice for antibody-drug conjugate (ADC) bioanalysis includes quantification of total antibody and antibody-conjugated drug. Here, we report a novel 2-in-1 approach for measuring total antibody and protease-cleavable conjugated drug Monomethyl Auristatin E (MMAE) concurrently. This allows for the determination of the DAR (Drug Antibody Ratio) for in vivo samples, with a 3-orders linear range based on total antibody concentration from 0.

DNA barcoding the bees (Hymenoptera: Apoidea) of Chile: species discovery in a reasonably well known bee fauna with the description of a new species of Lonchopria (Colletidae).

We compare the diversity of bees in the Chilean fauna as understood from traditional taxonomy-based catalogues with that currently known from DNA barcodes using the BIN system informed by ongoing morphology-based taxonomic research. While DNA barcode surveys of the Chilean bee fauna remain incomplete, it is clear that new species can readily be distinguished using this method and that morphological differentiation of distinct barcode clusters is sometimes very easy. We assess the situation in two genera in some detail.

The Bees among Us: Modelling Occupancy of Solitary Bees.

Occupancy modelling has received increasing attention as a tool for differentiating between true absence and non-detection in biodiversity data. This is thought to be particularly useful when a species of interest is spread out over a large area and sampling is constrained. We used occupancy modelling to estimate the probability of three phylogenetically independent pairs of native-introduced species [Megachile campanulae (Robertson)-Megachile rotundata (Fab.

Forecasting pollination declines through DNA barcoding: the potential contributions of macroecological and macroevolutionary scales of inquiry.

While pollinators are widely acknowledged as important contributors to seed production in plant communities, we do not yet have a good understanding of the importance of pollinator specialists for this ecosystem service. Determination of the prevalence of pollinator specialists is often hindered by the occurrence of cryptic species and the limitations of observational data on pollinator visitation rates, two areas where DNA barcoding of pollinators and pollen can be useful. Further, the demonstrated adequacy of pollen DNA barcoding from historical records offers opportunities to observe the effects of pollinator loss over longer timescales, and phylogenetic approaches can elucidate the historical rates of extinction of specialist lineages.

DNA barcoding as a useful tool in the systematic study of wild bees of the tribe Augochlorini (Hymenoptera: Halictidae).

Special care is needed in the delimitation and identification of halictid bee species, which are renowned for being morphologically monotonous. Corynura Spinola and Halictillus Moure (Halictidae: Augochlorini) contain species that are key elements in southern South American ecosystems. These bees are very difficult to identify due to close morphological similarity among species and high sexual dimorphism.

Comprehensive phylogeny, biogeography and new classification of the diverse bee tribe Megachilini: Can we use DNA barcodes in phylogenies of large genera?

Classification and evolutionary studies of particularly speciose clades pose important challenges, as phylogenetic analyses typically sample a small proportion of the existing diversity. We examine here one of the largest bee genera, the genus Megachile - the dauber and leafcutting bees. Besides presenting a phylogeny based on five nuclear genes (5480 aligned nucleotide positions), we attempt to use the phylogenetic signal of mitochondrial DNA barcodes, which are rapidly accumulating and already include a substantial proportion of the known species diversity in the genus.

Rapid quantification of a cleavable antibody-conjugated drug by liquid chromatography/tandem mass spectrometry with microwave-assisted enzymatic cleavage.

Antibody-drug conjugates (ADCs) play an increasingly important role for targeted cancer treatment. One class of ADCs has attracted particular interest in drug development. These ADCs employ a cleavable chemistry linkage for drugs and utilize the reduced interchain disulfide cysteine residues for conjugation.