Analysis of Human Stem Cell Transcription Factors.

Transcription factors , , , and are expressed in both human embryonic stem cells (hESCs) and cancer stem cells and they play a crucial role in maintaining characteristics of stemness such as self-renewal and pluripotency. This article evaluates the expression of variants of the main stem cell-specific transcription factors and critically and accurately with specific primers designed for identifying the most important variants that maintain stemness. We have examined four variants of along with a processed pseudogene and seven variants of in human teratocarcinoma cell lines (NTERA2D1, SuSa, GCT-27, and 833KE), hESCs, and ovarian cancer cells by reverse transcriptase-polymerase chain reaction.