A longitudinal study of the effect of temperature modification in full-scale anaerobic digesters - dataset combining 16S rDNA gene sequencing, metagenomics, and metabolomics data.

Data in this article provides detailed information on the microbial dynamics and degradation performances in two full-scale anaerobic digesters operated in parallel for 476 days. One of them was kept at 35 °C for the whole experiment, while the other was submitted to sub-mesophilic (25 °C) conditions between days 123 and 373. Sludge samples were collected from both digesters at days 0, 80, 177, 218, 281, 353, and 462.

Metataxonomics, metagenomics and metabolomics analysis of the influence of temperature modification in full-scale anaerobic digesters.

Full-scale anaerobic digesters' performance is regulated by modifying their operational conditions, but little is known about how these modifications affect their microbiome. In this work, we monitored two originally mesophilic (35 °C) full-scale anaerobic digesters during 476 days. One digester was submitted to sub-mesophilic (25 °C) conditions between days 123 and 373.

Pilot scale sulfur dioxide-ethanol-water fractionation of recycled wood to sugars, bioethanol, lignin and lignosulfonates: Carbohydrate balance.

Two grades of recycled wood (Waste Wood A and Waste Wood B) were fractionated on a pilot scale (800 BD kg) to monomeric sugars, lignin and lignosulfonates using SO-Ethanol-Water (AVAP®) technology, including pretreatment, separation of cellulosic and hemicellulosic streams, and saccharification. Carbohydrate mass balance was obtained through determination of poly-, oligo- and monosaccharides as well as sugar degradation products in process streams. High monosaccharide yields were obtained confirming laboratory scale findings.

New insights into the key microbial phylotypes of anaerobic sludge digesters under different operational conditions.

Analyses on bacterial, archaeal communities at family level and methane-production metabolism were conducted in thirteen full-scale and pilot-scale anaerobic sludge digesters. These digesters were operated at different conditions regarding solids concentration, sludge retention time, organic loading rate and feedstock composition, seeking to optimize digester capacity. Correlations between process parameters and identified microbial phylotypes were evaluated based on relative abundance of these phylotypes determined by Quantitative PCR and 16S rDNA amplicon sequencing.

Influence of phenylacetic acid pulses on anaerobic digestion performance and archaeal community structure in WWTP sewage sludge digesters.

The effect of phenylacetic acid (PAA) pulses on anaerobic digestion (AD) performance and archaeal community structure was evaluated in anaerobic digesters treating sewage sludge from a wastewater treatment plant (WWTP). Four pilot-scale continuous stirred tank reactors were set up at a full-scale municipal WWTP in Santiago de Chile, and fed with either primary or mixed sewage sludge. AD performance was evaluated by volatile fatty acid (VFA) and biogas production monitoring.

In situ microbial fuel cell-based biosensor for organic carbon.

The biological oxygen demand (BOD) may be the most used test to assess the amount of pollutant organic matter in water; however, it is time and labor consuming, and is done ex-situ. A BOD biosensor based on the microbial fuel cell principle was tested for online and in situ monitoring of biodegradable organic content of domestic wastewater. A stable current density of 282±23mA/m(2) was obtained with domestic wastewater containing a BOD(5) of 317±15mg O(2)/L at 22±2°C, 1.