Publications by authors named "Pablo Bora"
Article Synopsis
- Preimplantation mouse embryo development involves the differentiation of three key cell types: trophectoderm, primitive endoderm, and epiblast, which begins to spatially separate during the 8- to 16-cell stage.
- The generation of primary inner cell mass (ICM) cells is heavily influenced by the activation of the mTOR pathway at the 8-cell stage, impacting the translation of specific mRNA sequences.
- Inhibition of mTOR can reduce ICM cell numbers in early blastocysts, but this effect can be compensated later on, indicating a flexible development process in response to environmental changes.
Article Synopsis
- The study investigates the role of p38-MAPK in mouse preimplantation development, focusing on how it affects the differentiation of the inner cell mass (ICM) and primitive endoderm (PrE) by regulating RNA processing and translation.
- Researchers specifically examine DEAD-box RNA helicase 21 (DDX21), finding that its expression is crucial during blastocyst development and is associated with p38-MAPK activity.
- Results from knockdown experiments reveal that reduced DDX21 leads to significant defects in cell proliferation and differentiation, highlighting its importance in lineage-specific translation regulation in the early stages of mouse embryo development.
Article Synopsis
- * The study identifies that p38 mitogen-activated protein kinases (p38-MAPKs) are active early in blastocyst development, regulating essential processes like ribosome function and protein translation necessary for PrE differentiation.
- * Inhibiting p38-MAPK affects PrE development but can be partly reversed by activating mTOR, whereas similar effects from inhibiting the ERK pathway linked to FGF4 are not reversed by mTOR, highlighting p38-MAPK's unique role in PrE development. *
Article Synopsis
- The formation of the mouse blastocyst concludes preimplantation development, creating three main cell types: trophectoderm, primitive endoderm, and epiblast.
- Abnormal expression of a specific gene related to Hippo-signaling leads to issues with cell division and overall embryo cell count.
- Dysregulation also affects oocyte development, causing defects in cell division and failure in key molecular activation, but these issues can be reversed with the right protein expression, highlighting the gene's role in early cell development stages.
Article Synopsis
- - Maternal starvation during preimplantation development significantly affects placental and fetal growth, leading to long-term health issues according to the Developmental Origin of Health and Disease (DOHaD) hypothesis.
- - Research reveals that p38-mitogen activated kinases (p38-MAPK) are crucial for the differentiation of the primitive endoderm (PrE) in mouse embryos and how amino acid levels influence this process.
- - Findings suggest that while p38-MAPK helps manage cell number and lineage specification in response to amino acid availability, its inhibition without amino acids leads to severe developmental defects, highlighting its important regulatory role in early development and its implications for DOHaD.
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions.
View Article and Find Full Text PDFThe human genomic locus for the transcription factor TOX3 has been implicated in susceptibility to restless legs syndrome and breast cancer in genome-wide association studies, but the physiological role of TOX3 remains largely unknown. We found Tox3 to be predominantly expressed in the developing mouse brain with a peak at embryonic day E14 where it co-localizes with the neural stem and progenitor markers Nestin and Sox2 in radial glia of the ventricular zone and intermediate progenitors of the subventricular zone. Tox3 is also expressed in neural progenitor cells obtained from the ganglionic eminence of E15 mice that express Nestin, and it specifically binds the Nestin promoter in chromatin immunoprecipitation assays.
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