Expression, localization, and function of the thioredoxin system in diabetic nephropathy.

Excessive reactive oxygen species play a key role in the pathogenesis of diabetic nephropathy, but to what extent these result from increased generation, impaired antioxidant systems, or both is incompletely understood. Here, we report the expression, localization, and activity of the antioxidant thioredoxin and its endogenous inhibitor thioredoxin interacting protein (TxnIP) in vivo and in vitro. In normal human and rat kidneys, expression of TxnIP mRNA and protein was most abundant in the glomeruli and distal nephron (distal convoluted tubule and collecting ducts).

Electromyography and kinematics of the trunk during rowing in elite female rowers.

Purpose: The purpose of this study was to characterize the EMG of trunk muscles together with kinematics of the pelvis and the spine of elite female rowers during the rowing stroke.

Methods: Nine Rowing Canada national team candidates performed a 2000-m race simulation. EMG activity of spinal and pelvic extensor and flexor muscles and kinematic data of the pelvis and the spine were collected and analyzed during the period of peak force production.

Isolation and primary culture of human proximal tubule cells.

Primary cultures of renal proximal tubule cells (PTC) have been widely used to investigate tubule cell function. They provide a model system where confounding influences of renal haemodynamics, cell heterogeneity, and neural activity are eliminated. Additionally they are likely to more closely resemble PTC in vivo than established kidney cell lines, which are often virally immortalised and are of uncertain origin.

Efficacy of a florfenicol-flunixin meglumine combination product versus tulathromycin or ceftiofur crystalline free acid for the treatment of undifferentiated fever in feedlot calves.

In this field trial, a new combination product containing florfenicol and flunixin meglumine (FLOR-FM) was compared with commercially available products that contained only tulathromycin (TULA) or ceftiofur crystalline free acid (CCFA) for the treatment of undifferentiated fever (UF; rectal temperature >/=105.0 degrees F) in beef calves that received long-acting oxytetracycline at feedlot arrival. The overall mortality rate of the FLOR FM group (2.

The role of Sgk-1 in the upregulation of transport proteins by PPAR-{gamma} agonists in human proximal tubule cells.

Background: Cellular sodium and water transport are dysregulated in diabetes mellitus. Synthetic peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonists are currently used in the treatment of type 2 diabetes, but their use is limited by fluid retention. Recent data suggest that PPAR-gamma agonists stimulate distal tubular epithelial Na transport, potentially through the serine glucocorticoid kinase-1 (Sgk-1)-dependent regulation of the epithelial Na channel.

The influence of desk and display design on posture and muscle activity variability whilst performing information technology tasks.

Desk design and computer display height can affect posture and muscle activation during computer use. Amplitudes of postural variables and muscle activity during computer use do not explain the results from epidemiological studies of musculoskeletal discomfort and disorders related to computer use. The purpose of this study was to assess variability of posture and muscle activity during work with two computer display heights and book/paper, in conjunction with a curved desk designed to provide forearm support and a traditional, straight desk.

The status of the world's land and marine mammals: diversity, threat, and knowledge.

Knowledge of mammalian diversity is still surprisingly disparate, both regionally and taxonomically. Here, we present a comprehensive assessment of the conservation status and distribution of the world's mammals. Data, compiled by 1700+ experts, cover all 5487 species, including marine mammals.

DNA-based animal models of human disease: from genotype to phenotype.

Biomedical research utilizes animal models to elucidate human disease processes at the cellular and molecular level and for the development of new therapies. Traditionally, mammalian models have been limited to the mouse, primarily because of well characterized genetic lines and the ability to manipulate the genome to directly test hypotheses regarding causal mutations and disease phenotypes. The emerging availability of genome sequences of other mammals (bovine, canine, equine, feline, and porcine) now permits utilization of the mammal in which the phenotype best approximates the human condition.

Role of Kruppel-like factor 6 in transforming growth factor-beta1-induced epithelial-mesenchymal transition of proximal tubule cells.

Krüppel-like factor 6 (KLF6) is a DNA-binding protein containing a triple zinc-fingered motif and plays a key role in the regulation of cell proliferation, differentiation, and development. More recently it has been implicated in hepatic fibrosis via its binding to the transforming growth factor (TGF)-beta control element. In the kidney, epithelial-mesenchymal transition (EMT) is a major contributor to the pathogenesis of renal fibrosis, with TGF-beta1 being a key mediator of EMT.

High glucose induced endothelial cell growth inhibition is associated with an increase in TGFbeta1 secretion and inhibition of Ras prenylation via suppression of the mevalonate pathway.

Objective: Ras proteins are known to affect cellular growth and function. The influence of the prenylation status of Ras on the observed changes in endothelial cell growth under high glucose conditions has not previously been examined.

Methods: Human umbilical vein endothelial cells were exposed to normal or high glucose conditions for 72 h.

West Nile virus in the Americas.

West Nile virus causes sporadic disease in the Eastern hemisphere that is often asymptomatic or mild, whereas in the Western hemisphere, West Nile virus has been associated with illness and profound mortality in many avian species. West Nile virus might have been transported to North America by an infected mosquito or the virus could have entered within a vertebrate host like a bird. Although the most important method of West Nile virus transmission is by Culex species mosquitoes, additional modes of transmission have been identified.

A comparison of florfenicol and tulathromycine for the treatment of undifferentiated fever in feedlot calves.

The purpose of this study was to compare the relative cost-effectiveness of florfenicol with that of tulathromycin for treatment of undifferentiated fever (UF) in feedlot calves at ultra-high risk of developing UF that receive metaphylactic tulathromycin on arrival at the feedlot. Calves that received therapeutic florfenicol had lower overall mortality (P=.045) and bovine respiratory disease mortality (P=.

A randomised, cross-over study comparing injection site pain with subcutaneous epoetin beta and subcutaneous darbepoetin alfa in patients with chronic kidney disease.

Objective: To compare injection site pain of subcutaneous (sc) epoetin beta and darbepoetin alfa in adult patients with chronic kidney disease.

Research Design And Methods: This was a multi-centre, randomised, two-arm, single-blind, cross-over study. Patients were randomised to receive weekly sc darbepoetin alfa 30 mug or weekly sc epoetin beta 6000 IU for 2 weeks and were then crossed over to the alternative treatment for 2 weeks.

Perinucleolar compartment prevalence is a phenotypic pancancer marker of malignancy.

Background: The perinucleolar compartment (PNC) is a subnuclear structure localized at the nucleolar periphery. Previous studies using breast cancer as a model system demonstrated that PNC prevalence (the percentage of cells with 1 or more PNC) increased with disease progression and was associated with poor patient outcomes.

Methods: To evaluate the validity of developing PNC prevalence as a novel pancancer prognostic marker, the authors investigated whether PNC prevalence was correlated with malignancy in a spectrum of tissue types and evaluated its selective association with malignancy under various experimental conditions.

The effect of forearm support on children's head, neck and upper limb posture and muscle activity during computer use.

Use of computers by children has increased rapidly, however few studies have addressed factors which may reduce musculoskeletal stress during computer use by children. This study quantified the postural and muscle activity effects of providing forearm support when children used computers. Twelve male and 12 female children (10-12 years) who regularly used computers were recruited.

A comparison of posture and muscle activity during tablet computer, desktop computer and paper use by young children.

Computers are now widely used by children. Tablet computers are becoming widely available and promoted for use by school children. The primary objective of this study was to compare the posture and muscle activity of children using a tablet computer to the posture and muscle activity of children using a desktop computer and paper technology.

Children's posture and muscle activity at different computer display heights and during paper information technology use.

Objective: The 3-D posture and muscle activity in the neck and upper limb were assessed in children using high-, mid-, and book-level displays, which correspond to working conditions frequently observed when children interact with computers or books and paper.

Background: The 3-D posture and muscle activity of children reading and inputting data with computers and paper had not been previously assessed.

Methods: Twenty-four children aged 10 to 12 years and of normal height performed an interactive task involving reading from a book and writing on paper or reading from a computer display and inputting data using a mouse and keyboard.

Single and multiple-dose pharmacokinetics of tepoxalin and its active metabolite after oral administration to rabbits (Oryctolagus cuniculus).

The anti-inflammatory agent, tepoxalin, was administered to eight healthy 6-month-old female New Zealand white rabbits once daily at an oral dose of 10 mg/kg. Blood samples were obtained immediately before and at 0.25, 0.

Increased renal gene transcription of protein kinase C-beta in human diabetic nephropathy: relationship to long-term glycaemic control.

Aims/hypothesis: Activation of protein kinase C (PKC) isoforms has been implicated as a central mediator in the pathogenesis of diabetic nephropathy. Although high glucose levels stimulate catalytic activity of PKC, the effects of high glucose levels on the expression of genes encoding PKC isoforms are unknown. We sought to determine whether in addition to activation, diabetes may lead to increased transcription of two PKC isoforms that have been implicated in the pathogenesis of diabetic nephropathy, PKC-alpha and PKC-beta.

Children have less variable postures and muscle activities when using new electronic information technology compared with old paper-based information technology.

Children now have considerable exposure to new information technologies (IT) such as desktop computers. A reported association between computer use and discomfort in children has prompted concerns about the musculoskeletal stresses associated with computer use. There were no detailed data on children reading and writing, nor any evidence on the variability of postures and muscle activity whilst children use IT.

The role of SGK-1 in angiotensin II-mediated sodium reabsorption in human proximal tubular cells.

Background: The role of angiotensin II (Ang II) in mediating excessive sodium reabsorption in diabetic nephropathy is recognized. Serine-glucocorticoid kinase-1 (SGK-1) increases sodium-hydrogen exchanger-3 (NHE3) expression and is known to be upregulated in in vitro and in vivo models of diabetic nephropathy. However, a link between Ang II and SGK-1 in diabetic nephropathy has not been established.

A context of uncertainty: how context shapes nurses' research utilization behaviors.

Context has often been cited as a significant factor in shaping research utilization behavior, yet scholars have been unable to provide specific detail as to how and why it is important. From an ethnographic study of research utilization in a pediatric intensive care unit, we determined that the primary characteristic of this nursing unit was uncertainty. We identified four major sources of uncertainty: (1) the precarious status of seriously ill patients, (2) the inherent unpredictability of nurses' work, (3) the complexity of teamwork in a highly sophisticated hospital environment, and (4) a changing management.

The role of nursing unit culture in shaping research utilization behaviors.

We conducted a focused ethnography of a pediatric critical care unit to examine the role of nursing unit culture related to research utilization. Four significant aspects of the unit culture shaped nurses' research utilization. A hierarchical structure of authority, routinized and technology-driven work at the bedside, a workplace ethos that discouraged innovation, and an emphasis on clinical experience acted together to teach nurses both that they were to do as they were told and that they were not expected to use research.

Pure red cell aplasia induced by erythropoiesis-stimulating agents.

Pure red cell aplasia in patients who are treated for anemia of chronic kidney disease with erythropoiesis-stimulating agents such as epoetin was first reported in 1998. Although the incidence of pure red cell aplasia peaked in 2002, it remains important for nephrologists to know how to investigate a suspected case of pure red cell aplasia and how to identify other causes of hyporesponsiveness to erythropoiesis-stimulating agents, which account for the vast majority of such cases. The authors reviewed the current status of information in the literature and drew on their personal experiences with patients regarding the diagnosis and management of epoetin-induced pure red cell aplasia.