Redirecting glucose flux during in vitro expansion generates epigenetically and metabolically superior T cells for cancer immunotherapy.

Cellular therapies are living drugs whose efficacy depends on persistence and survival. Expansion of therapeutic T cells employs hypermetabolic culture conditions to promote T cell expansion. We show that typical in vitro expansion conditions generate metabolically and functionally impaired T cells more reliant on aerobic glycolysis than those expanding in vivo.

Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study.

Introduction: The World Health Organization (WHO) points out that infection by enteroparasites can affect ~3.5 billion people around the world. Hemodialysis (HD) patients may be more susceptible to infections by opportunistic pathogens due to impaired immune function.

COVID-19 Increases the Risk of New Myocardial Infarction in Patients with Old Myocardial Infarction: A Retrospective Observational Study.

Background: We aimed to investigate the incidence of new acute myocardial infarction (AMI), in patients with Coronavirus disease (COVID-19) who had old MI. We hypothesized that COVID-19 increases the rate of repeated AMI in this population regardless of age and gender.

Methods: A retrospective analysis was conducted for adult patients admitted with COVID-19 and developed thromboembolic event (TEE) in 2020.

UTSA-16(Zn) for SO detection: elucidating the fluorescence mechanism.

In this study, the potential of the metal-organic framework UTSA-16(Zn) as a fluorescence detector for SO is explored. The material was synthesized and characterized by powder X-ray diffraction (PXRD), infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), and its fluorescence behavior was analyzed before and after SO exposure. A significant decrease in fluorescence intensity was observed and a LOD of 1.

Jatoba ( L.) Pod Residue: A Source of Phenolic Compounds as Valuable Biomolecules.

This study aimed at investigating the chemical composition and a selected group of bioactivities of jatoba ( L.) pod residue. An aqueous extract (deionized water; AE) and a hydroethanolic extract (ethanol: deionized water, 70:30 /; ETOH) were obtained via maceration.

Dysfunction of exhausted T cells is enforced by MCT11-mediated lactate metabolism.

CD8 T cells are critical mediators of antitumor immunity but differentiate into a dysfunctional state, known as T cell exhaustion, after persistent T cell receptor stimulation in the tumor microenvironment (TME). Exhausted T (T) cells are characterized by upregulation of coinhibitory molecules and reduced polyfunctionality. T cells in the TME experience an immunosuppressive metabolic environment via reduced levels of nutrients and oxygen and a buildup of lactic acid.

Record-Breaking HS Capture and ppm-Level Sensing with a Chemically Stable Porous Organic Cage.

The first experimental investigation of a porous organic cage (POC) for the challenging task of HS capture is reported. The N-containing cage molecular material, a tertiary amine POC (6FT-RCC3), demonstrates the highest HS (hydrogen sulfide) capture (record capacity) for a porous material at room temperature and atmospheric pressure (20.6 mmol HS g; 25 HS molecules per cage) combined with excellent reversibility for at least five adsorption-desorption cycles.

Effective BPA degradation in water: the integration of bimetallic UiO-66 Ce-Zr.

In this work, a bimetallic MOF UiO-66 Ce-Zr to degrade bisphenol A (BPA) in water was synthesised. The material exhibited a remarkable degradation efficiency of 84.3% under UV irradiation for 240 minutes.

Available Treatments and Adjunctive Therapies for Polycystic Ovarian Syndrome (PCOS) Patients of Reproductive Age: A Scoping Review.

Polycystic ovarian syndrome (PCOS) impacts the health of women worldwide. It is a condition consisting of dysfunctional cystic ovaries resulting in hormonal imbalance. Many women have symptoms such as infertility, increased production of androgens, and insulin resistance.

Phosphoester bond hydrolysis by a discrete zirconium-oxo cluster: mechanistic insights into the central role of the binuclear Zr-Zr active site.

Effective degradation of non-natural phosphate triesters (PTs) widely used in pesticides and warfare agents is of paramount relevance for human and environmental safety, particularly under acidic conditions where they are highly stable. Here, we present a detailed reactivity and mechanistic study pioneering discrete {ZrO} clusters, which are commonly employed as building blocks for Zr-MOFs and as non-classical soluble coordination compounds for the degradation of PTs using the pesticide ethyl paraoxon as a model. Combined computational studies, mechanistic experiments, and EXAFS analysis show that the reactivity of these clusters arises from their Zr-Zr bimetallic sites, which hydrolyze ethyl paraoxon under acidic conditions through an intramolecular pathway.

Properties and kinetic behavior of free and immobilized laccase from Oudemansiella canarii: Emphasis on the effects of NaCl and NaSO on catalytic activities.

Studies have highlighted the great potential of Oudemansiella canarii laccase in degrading synthetic dyes for reducing their toxicity. Immobilization of enzymes improves usability in degradation processes and the present work succeeded in immobilizing this laccase onto MANAE-agarose. Immobilization improved pH, thermal, and storage stabilities.

Phase transitions in random circuit sampling.

Undesired coupling to the surrounding environment destroys long-range correlations in quantum processors and hinders coherent evolution in the nominally available computational space. This noise is an outstanding challenge when leveraging the computation power of near-term quantum processors. It has been shown that benchmarking random circuit sampling with cross-entropy benchmarking can provide an estimate of the effective size of the Hilbert space coherently available.

Gastrointestinal digestion of yerba mate, rosemary and green tea extracts and their subsequent colonic fermentation by human, pig or rat inocula.

Polyphenolic compounds are common constituents of human and animal diets and undergo extensive metabolism by the gut microbiota before entering circulation. In order to compare the transformations of polyphenols from yerba mate, rosemary, and green tea extracts in the gastrointestinal tract, simulated gastrointestinal digestion coupled with colonic fermentation were used. For enhancing the comparative character of the investigation, colonic fermentation was performed with human, pig and rat intestinal microbiota.

Effects of resveratrol on rheumatic symptoms and hepatic metabolism of arthritic rats.

Objectives: Resveratrol has been studied as a potential agent for treating rheumatic conditions; however, this compound suppresses glucose synthesis and glycogen catabolism when infused in perfused livers of both arthritic and healthy rats. This study investigated the effects of oral administration of resveratrol on inflammation and liver metabolism in rats with arthritis induced by Freund's adjuvant, which serves as rheumatoid arthritis model.

Methods: Holtzman rats, both healthy and exhibiting arthritic symptoms, were orally treated with resveratrol at doses varying from 25 to 500 mg/kg for a 5-day period preceding arthritis induction, followed by an additional 20-day period thereafter.

Olive Oil (Royal Cultivar) from Mill Obtained by Short Time Malaxation and Early Ripening Stage.

The olive oil from the Royal cultivar has not been studied in depth, especially its relationship between analytical and sensory parameters. Currently, it is a minority cultivar, but due to its excellent organoleptic properties, it is constantly growing. The research objective is to obtain excellent-quality olive oil from the Royal cultivar at an industrial extraction plant and characterize the oil sensory and analytically.

A Biologic With Otologic Consequences: Analysis of Hearing Loss and Teprotumumab Using the FDA Adverse Event Reporting System.

Objectives: To evaluate otologic adverse reactions (OARs), including hearing loss (OARs-HL) among patients taking teprotumumab, a new biologic approved for the treatment of active thyroid eye disease, using publicly available pharmacovigilance reporting data.

Study Design: Retrospective database review.

Methods: The Food and Drug Administration Adverse Events Reporting System (FAERS) was queried for cases involving teprotumumab from 2020Q1 to 2023Q1.

Utility of an Alpha-1 Adrenergic Agonist in the Management of Chylothorax: A Case Series and Management Algorithm.

Unlabelled: Chylothorax is the accumulation of lymphatic fluid (chyle) within the pleural space. There are multiple causes, including traumatic and non-traumatic mechanisms. Trauma can cause disruption of the thoracic duct either by direct damage or indirect crushing or avulsion mechanisms.

Room-temperature synthesis of bimetallic ZnCu-MOF-74 as an adsorbent for tetracycline removal from an aqueous solution.

The novel bimetallic MOF, ZnCu-MOF-74, has been evaluated for the remediation of tetracycline-contaminated water. ZnCu-MOF-74 was obtained at room temperature, avoiding high pressure and temperature. ZnCu-MOF-74 exhibited chemical stability in the 4-8 pH range.

Early major fracture care in polytrauma-priorities in the context of concomitant injuries: A Delphi consensus process and systematic review.

Background: The timing of major fracture care in polytrauma patients has a relevant impact on outcomes. Yet, standardized treatment strategies with respect to concomitant injuries are rare. This study aims to provide expert recommendations regarding the timing of major fracture care in the presence of concomitant injuries to the brain, thorax, abdomen, spine/spinal cord, and vasculature, as well as multiple fractures.