Cloning and expression of the Epstein-Barr virus-encoded dUTPase: patients with acute, reactivated or chronic virus infection develop antibodies against the enzyme.

The gene encoding the Epstein-Barr virus (EBV)-specific dUTPase was amplified from virus DNA by PCR. The active enzyme was expressed in Escherichia coli and in insect cells as a non-fusion protein. The protein from E.

Identification of tyrosine as a functional residue in the active site of Escherichia coli dUTPase.

dUTP nucleotidohydrolase (dUTPase, EC 3.6.1.