MRSA carriage in a tertiary governmental hospital in Thailand: emphasis on prevalence and molecular epidemiology.

We investigated prevalence and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) in a case-control study performed in a 900-bed tertiary governmental healthcare facility in Bangkok, Thailand. Multivariate unconditional logistic regression was used to identify risk profiles for MRSA carriage. Phage typing, pulsed-field gel electrophoresis (PFGE), polymorphisms of the coa and spa genes, hypervariable region (HVR) of SCCmec, multi-locus sequence typing (MLST), and identification of ST30/ST8 mosaic chromosome by heteroduplex-polymerase chain reaction (heteroduplex-PCR) were used to demonstrate a clonal relationship.

Cross-infection of gentamicin-methicillin-resistant Staphylococcus aureus in a male surgical ward at Rajavithi General Hospital.

Between January and December 1987, gentamicin-methicillin-resistant strains of Staphylococcus aureus (GMRSA) were isolated from 7 patients in a male surgical ward at Rajavithi General Hospital. Six patients developed significant infection which included sepsis (2), pneumonia (1), infection in the eye, ear and wound (1), wound infection (2), and one patient had GMRSA isolated from his sputum. The strains were untypable with standard phage type and were resistant to methicillin, gentamicin, amikacin, kanamycin, streptomycin, tetracycline, erythromycin and chloramphenicol, but susceptible o vancomycin and cotrimoxazole.