Trichlorofluoromethane, in concentrations of 80, 400, 2 000, 10 000 and 50 000 ppm, was administered to rats of both sexes (Sprague Dawley) by inhalation exposure.2 000 ppm in air (= 11 200 mg/m(3)) amount to twice the MAK value of 1 000 ppm. At exposure times of 4 hours this corresponds to the MAK value defined for an 8 hour workday.
View Article and Find Full Text PDFBenzo[a]pyrene, benzo[b]fluoroanthene and dibenzo[a,h]anthracene dissolved in a 1:2 mixture of dimethylsulphoxide (DMSO) and water were administered to two groups of female mice, each group containing 15 mice. The doses were administered orally (via gavage) at the respective rates of 1 and 100 micrograms kg-1 body weight five times per week for a period of 9 weeks. The influence of the polycyclic aromatic hydrocarbons (PAHs) was determined using the following methods: determination of DNA-PAH adducts, of chromosome injuries (micronucleus test), of induction of repair using the unscheduled DNA synthesis (UDS) test, and by examination of the DNA structure after nucleoid sedimentation.
View Article and Find Full Text PDFThe induction of DNA-synthesis in non-S-phase cells is a very sensitive measure of a preceding damage of DNA. Usually, in an in vivo-in vitro test (treatment of an animal, incorporation of H3-thymidine in a cell suspension) the damaging of DNA takes place hours to days before the evaluation. In this case, the time course of the UDS-induction after a single dose of 1 Gy gamma irradiation was observed over a long period of time (21 months).
View Article and Find Full Text PDFWe investigated the influence of nicotine and its metabolites on semiconservative DNA synthesis, DNA repair processes, and the rejoining of DNA strand breaks. Using a combined treatment with nicotine and gamma irradiation and counting the number of sister chromatid exchanges, we evaluated the action of nicotine on the whole DNA repair process, especially examining the possibility that DNA is damaged by the action of nicotine. All tests were performed on HeLa cells and verified with human lung fibroblasts.
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