Publications by authors named "P W Sanger"
Article Synopsis
- Clinical imaging techniques like PET combined with CT are becoming more common in biomedical research with small animals, but handling radioactive substances requires strict regulations and training to ensure safety.
- This paper outlines the requirements set by the Radiation Protection Act and associated regulations, with a focus on practical steps in PET/CT imaging, from receiving radiopharmaceuticals to waste disposal.
- An analysis of radiation doses over four years in a small animal imaging facility showed that personnel exposure is generally lower than in human PET/CT imaging, remaining well within legal limits.
The insecticidal toxin complex (Tc) proteins are produced by several insect-associated bacteria, including Yersinia enterocolitica strain W22703, which oscillates between two distinct pathogenicity phases in invertebrates and humans. The mechanism by which this high-molecular-weight toxin is released into the extracellular surrounding, however, has not been deciphered. In this study, we investigated the regulation and functionality of a phage-related holin/endolysin (HE) cassette located within the insecticidal pathogenicity island Tc-PAI of W22703.
View Article and Find Full Text PDFArticle Synopsis
- The bacterial strain Yersinia enterocolitica W22703 is highly toxic to the Greater wax moth larvae and significantly proliferates in these hosts over six days.
- Deletion mutants lacking specific genes, tcaA and tccC, showed different behaviors, confirming TcaA as a critical component for membrane binding and TccC as a key cell toxin.
- The infection process involves initial colonization of the midgut, followed by invasion of the insect's hemolymph, leading to altered hemocyte morphology and a reprogramming of the pathogen's cellular functions to utilize the insect's nutrients.
A 70-year-old male patient was admitted to our dermatology outpatient clinic with newly developed personality changes and signs of hypoxemia. His anti-p200 Pemphigoid was treated with Dapsone for a few weeks. Due to generalized tonic-clonic seizure with a subsequent Glasgow Coma Scale of 5 points and a peripheral oxygen saturation not exceeding 88% under conditions of high-flow nasal cannula, he was intubated by the emergency team and transferred to the intensive care unit.
View Article and Find Full Text PDFThe genus comprises pathogens that can adapt to an environmental life cycle stage as well as to mammals. strain W22703 exhibits both insecticidal and nematocidal activity conferred by the tripartite toxin complex (Tc) that is encoded on the 19-kb pathogenicity island Tc-PAI All genes follow a strict temperature regulation in that they are silenced at 37°C but activated at lower temperatures. Four highly conserved phage-related genes, located within the Tc-PAI , were recently demonstrated to encode a biologically functional holin-endolysin gene cassette that lyses its own host W22703 at 37°C.
View Article and Find Full Text PDF