Reactivities of a monoclonal antibody directed against an immunosuppressive molecule in boar seminal plasma.

Using a monoclonal antibody (LIS-4) to an immunosuppressive factor isolated from boar vesicular gland secretion it was determined that this gland secretes a tissue-specific immunosuppressive molecule that is absorbed onto the acrosome of spermatozoa during ejaculation. Absorption of the immunosuppressive molecule onto murine embryos at the 2-, 4-, 8-cell, morula and blastocyst stages in vitro was evaluated by indirect immunofluorescence. In vivo absorption was detected on the zona pellucida of murine embryos obtained from oviducts injected with the immunosuppressive molecule.

Immunosuppressive factor in cow follicular fluid.

The activity of the immunosuppressive fraction and proteinase inhibitor isolated from cow follicular fluid was investigated. The immunosuppressive factor was separated from the accompanying proteinase inhibitor by Sepharose 6B and Sephacryl S-200 column chromatography. In vitro, the fraction significantly reduced mitogen-induced lymphocyte proliferation.

Lack of effect of boar seminal vesicle fluid immunosuppressor factor on embryo development.

The effects of an immunosuppressive factor (ISF) isolated from boar seminal vesicle fluid on in vitro and in vivo mouse development were investigated. It was found that the zona pellucida of porcine and mouse oocytes pre-incubated with ISF reacted in indirect immunofluorescence with antibodies against ISF. Further results indicated that the boar ISF had no effect on embryo development.

[The effect of selected antibiotics on microorganisms contaminating boar ejaculate].

The occurrence of microorganisms, including their total counts in boar native ejaculates, was investigated in two stages; the objective of this investigation also was to determine contamination after the sperms were treated with diluents containing the antibiotics ampicillin, gentamycin, apramycin, cefoxitin, or antibiotic combinations penicillin + streptomycin, ampicillin + cefoxitin, gentamycin + cefoxitin and ampicillin + gentamycin. The representation of bacterial species and total counts of microbes in 1 ml diluted sperm stored at a temperature of about 18 degrees C were determined in 24, 48 and 72 h after dilution. The microorganisms were cultivated from all native ejaculates.