Gold Nanoprism Enhanced SERS Aptasensor for Simultaneous Detection of Thrombin and VEGF.

Sensitive detection of disease-specific biomarkers with high accuracy is crucial for early diagnosis, therapeutic monitoring, and understanding underlying pathological mechanisms. Traditional methods, such as immunohistochemistry and enzyme-linked immunosorbent assays (ELISA), face limitations due to the complex and expensive production of antibodies. In this context, aptamers, short oligonucleotides with advantages like easy synthesis, low cost, high specificity, and stability, have emerged as promising alternatives for biomolecular sensing.

Uncovering Astrocyte Morphological Dynamics Using Optical Diffraction Tomography and Shape-Based Trajectory Inference.

Astrocytes, integral components of the central nervous system, are increasingly recognized for their multifaceted roles beyond support cells. Despite their acknowledged importance, understanding the intricacies of astrocyte morphological dynamics remains limited. Our study marks the first exploration of astrocytes using optical diffraction tomography (ODT), establishing a label-free, quantitative method to observe morphological changes in astrocytes over a 7-day in-vitro period.

Comparison of E-Cadherin Expression in Oral Verrucous Carcinoma and Normal Oral Mucosa: An Immunohistochemical Study.

Introduction Oral verrucous carcinoma (OVC), a low-grade variation of oral squamous cell carcinoma (OSCC), is distinguished by endophytic development and a pebbly, mammillated surface. OVC, often referred to as Ackerman's tumor, has been known to involve lymph nodes but rarely spreads to regional and distant locations; when the primary tumor grows, it frequently involves surrounding tissues. Histopathologically, it has a thicker basement membrane, many reduplications, and a large area of inflammatory infiltration that resembles OSCC.

Functional Outcomes Following Partial Osteotomy of the Calcaneal Tuberosity for Haglund's Syndrome.

Background Haglund's syndrome, a common cause of pain in the posterior heel that consists of painful swelling of the local soft tissues and prominence of the posterosuperior calcaneal projection, presents significant challenges in treatment, particularly when conservative management fails. This study evaluates the functional outcomes following oblique partial excision of the posterosuperior portion (calcaneal tuberosity osteotomy) of the calcaneus for Haglund's syndrome. Methods A cohort of 30 patients, aged 18 years and older, with persistent heel pain unresponsive to conservative treatments, underwent partial osteotomy using a medial or lateral approach.

To Acquire or Not to Acquire: Evaluating Compressive Sensing for Raman Spectroscopy in Biology.

Raman spectroscopy has revolutionized the field of chemical biology by providing detailed chemical and compositional information with minimal sample preparation. Despite its advantages, the technique suffers from low throughput due to the weak Raman effect, necessitating long acquisition times and expensive equipment. This limitation is particularly acute in time-sensitive applications like bioprocess monitoring and dynamic studies.