Publications by authors named "P VEGH"
Modular DNA Construct Design for High-Throughput Golden Gate Assembly.
Methods Mol Biol
October 2024
Article Synopsis
- Golden Gate cloning allows for the efficient assembly of DNA parts into custom genetic constructs, thanks to its "one-pot" reaction setup.
- This method supports high-throughput automation, making it possible to create thousands of constructs simultaneously.
- A crucial challenge is the design of these constructs, which can be complex and error-prone if done manually, so this chapter introduces a computational workflow to automate and streamline the design process.
Article Synopsis
- * The Edinburgh Genome Foundry has implemented a new quality control method using Oxford Nanopore sequencing technology, along with a software pipeline for detailed analysis of DNA constructs.
- * The developed protocol allows for rapid validation of plasmids, making it a valuable resource for researchers in genetics, synthetic biology, and sequencing fields.
As redesigning organisms using engineering principles is one of the purposes of synthetic biology (SynBio), the standardization of experimental methods and DNA parts is becoming increasingly a necessity. The synthetic biology community focusing on the engineering of has been in the foreground in this area, conceiving several well-characterized SynBio toolkits widely adopted by the community. In this review, the molecular methods and toolkits developed for are discussed in terms of their contributions to the required standardization efforts.
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- Tissue mononuclear phagocytes (MNP) play a crucial role in detecting pathogens and presenting antigens, with a focus on HIV transmission to CD4 T cells.
- Previous studies primarily targeted epithelial MNPs, but this research highlights the importance of sub-epithelial MNPs, especially in the context of mucosal trauma and inflammation linked to HIV transmission.
- The study identifies two specific subsets of sub-epithelial MNPs—CD14CD1c monocyte-derived dendritic cells and langerin-expressing conventional dendritic cells 2 (cDC2)—that are key in taking up HIV, becoming infected, and facilitating its transmission to CD4 T cells.
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic dermatitis and psoriasis. We leveraged these datasets to compare cell states across development, homeostasis, and disease.
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