Synergistic effect of ethyl-6-O-decanoyl glucoside and chlorhexidine in contact lens disinfection.

Contact lenses with adherent Serratia marcescens and Pseudomonas aeruginosa could not be sterilised either with polyaminopropyl biguanide or with chlorhexidine (CHX) commercial solutions meant for contact lens disinfection, even after treatment lasting 12 h. Ethyl-6-O-decanoyl glucoside (EDG) with chlorhexidine enhanced the killing significantly. The same was also found to be the case for dispersed cells of P.

Bioluminescent assay of D-3-hydroxybutyrate in serum.

An enzymatic assay of D-3-hydroxybutyrate in which the hydroxybutyrate dehydrogenase reaction is coupled to the bacterial oxidoreductase-luciferase system is described. The bioluminescent assay is based on either, end-point, or on initial velocity measurements. This simple and rapid assay requires a single serum sample of 10 microliters.

A rapid and simple method for evaluation of serum opsonic capacity against Bordetella pertussis by chemiluminescence.

Serum opsonic capacity against Bordetella pertussis was studied by using quantitative chemiluminescence (CL) which is known to have several advantages over conventional methods in the evaluation of opsonization and phagocytosis. For opsonization, sera from whooping cough patients or from controls were incubated with killed B. pertussis cells at 37 degrees C for 30 min.