HPLC analysis of tonka bean extracts.

Based on the results of GC-MS analysis, an HPLC procedure was developed for the determination of coumarin, dihydrocoumarin, melilotic acid (o-dihydrocoumaric acid), methyl melilotate, ethyl melilotate, 5-hydroxymethylfurfural and o-coumaric acid which are characteristic compounds of tonka beans. The presence of melilotic acid and ethyl melilotate in tonka beans has not been reported previously. Following HPLC analysis of tonka bean extracts produced in the laboratory and one sample of commercially available tonka bean absolute, evidence was provided that solvents used for production are decisive for the kind and amount of the compounds detected.

[Infrared spectroscopic determinations of mannitol].

Three different infrared-spectra of Mannit were obtained according to unused Mannit, melted Mannit, and the crystallized form out of methanol. The melting points of the three Mannit-modifications were in accordance 166.5 degrees C.

[Biotransformation of zipeprol (Mirsol) in humans. Gas chromatographic/mass spectrometric studies, using ammonia as a selective reactant gas (author's transl)].

After oral application of Zipeprol (Mirsol) the unchanged drug and nine degradation products were detected in human urine; six of them could be identified. All identified metabolites were detected in the alkaline urine fraction; after acid hydrolysis N-(2-hydroxyethyl)-piperazine was found as an artifact. With respect to the presented results, biotransformation of Zipeprol in man mainly follows three degradation routes: 1.

[On the metabolism of eprazinone (author's transl)].

After oral application of 3-[4-(beta-ethoxyphenethyl)-1-piperazinyl]-2-methylpropiophenone dihydrochloride (eprazinone, Eftapan) together with the unchanged drug, five degradation products were detected and subsequently identified by gas liquid chromatography/mass spectrometry in human urine. With respect to the presented results, eprazinone is degraded by various routes in man: 1. hydrolysis which leads to metabolites M1 and M4, and 2.