Publications by authors named "P Tijssen"
Article Synopsis
- - Parvoviruses are typically characterized by a monopartite ssDNA genome and specific structural proteins, but a new discovery has revealed a bipartite genome parvovirus, Acheta domesticus segmented densovirus (AdSDV), found in house crickets and exhibiting pathogenic traits.
- - AdSDV features its non-structural (NS) and structural (VP) protein cassettes spread across two different genome segments, with one segment having gained a new phospholipase A2-encoding gene through recombination, contributing to its complex transcription profile.
- - Structural analyses using cryo-electron microscopy show that AdSDV packages one genome segment per virus particle, with a unique mechanism that involves its VP
Article Synopsis
- * Serpentine AAV (SAAV), which does not spread in primate populations, is explored as a potential solution; the study investigates its structural properties, receptor interactions, and ability to evade specific antibodies using cryo-EM and various assays.
- * Findings reveal that SAAV primarily binds to terminal sialic acid, similar to some other AAV types, and while it can evade certain mouse antibodies, about 25% of human sera still recognize it, indicating possible challenges for its use in gene delivery.
The giant tiger prawn () is a decapod crustacean widely reared for human consumption. Currently, viruses of two distinct lineages of parvoviruses (PVs, family ; subfamily ) infect penaeid shrimp. Here, a PV was isolated and cloned from Vietnamese specimens, designated metallodensovirus (PmMDV).
View Article and Find Full Text PDFFemtosecond (fs) laser irradiation techniques are emerging tools for inactivating viruses that do not involve ionizing radiation. In this work, the inactivation of two bacteriophages representing protective capsids with different geometric constraints, that is, the near-spherical MS2 (with a diameter of 27 nm) and the filamentous M13 (with a length of 880 nm) is compared using energetic visible and near-infrared fs laser pulses with various energies, pulse durations, and exposure times. Intriguingly, the results show that inactivation using 400 nm lasers is substantially more efficient for MS2 compared to M13.
View Article and Find Full Text PDFIrradiation of femtosecond (fs) pulse lasers in the visible and near-infrared ranges have been proposed as a promising approach for inactivating viruses. However, in order to achieve significant virus inactivation, past works have required relatively long irradiation times (1 hour or longer), even for small volumes. Given its advantages compared with other techniques, there is an urgent need to shorten the time required to inactivate viruses using fs laser technology.
View Article and Find Full Text PDF