Publications by authors named "P Thuwanut"
Ovarian tissue cryopreservation and transplantation has emerged as a promising fertility preservation technique for individuals facing premature ovarian insufficiency due to various medical conditions or treatments. Xenotransplantation, involving the transplantation of ovarian tissue into animal hosts, has played a pivotal role in refining ovarian tissue cryopreservation and transplantation techniques and addressing key challenges. This review provides a comprehensive overview of the current landscape of ovarian tissue xenotransplantation research, focusing on its applications in investigating ovarian biology, optimizing ovarian tissue cryopreservation and transplantation protocols, and assessing safety concerns.
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- The study explored how fibrin-based hydrogel encapsulation impacted ovarian tissue quality and cell survival after freezing
- The research involved four groups of ovarian cortex from patients aged 44-47, comparing fresh tissue, uncapsulated tissue, and two encapsulated conditions (with and without VEGF)
- Key findings showed that encapsulation helped maintain signaling pathways important for cell survival, although some unfavorable effects on certain expression levels were noted, suggesting a need for further research to enhance this technique for fertility preservation.
This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2).
View Article and Find Full Text PDFOocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio.
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- The study explored how encapsulating ovarian tissue from domestic cats in a biodegradable hydrogel affects its ability to survive cryopreservation and maintain follicle quality during culture.
- The experiments involved comparing ovarian tissues with hydrogel encapsulation to controls, assessing factors like follicle viability and morphology after slow freezing and vitrification.
- Results showed that hydrogel encapsulation improved follicle survival and quality, suggesting potential methods for enhancing human fertility preservation through better cryopreservation techniques.