[Cultivation of cells in a medium with blood hydrolysate].

Use was made of fresh blood of swine, diluted with an equal amount of distilled water to produce a protein hydrolysate. Enzyme hydrolysis was effected through extracellular alkaline protease produced by stain DI of Bacillus subtilis. The cell lines BHK-21, PK-15 and spzv were cultured in a nutrient medium containing 0.

[Differentiation of foot-and-mouth disease viruses by counterimmunoelectrophoresis].

Studied were the opportunities for employing the method of counter immunoelectrophoresis (CIE) to differentiate F. M. D.

[Demonstration of specific VIA antibodies in foot-and-mouth disease].

Experiments were carried out to obtain a specific VIA antigen of F.M.D.

[Experiments to purify and concentrate the foot-and-mouth disease virus].

Experiments were carried out for the purification and concentration of the foot-and-mouth disease virus. For the purpose the virus suspensions were treated with chloroform and 8 per cent polyethylene glycol. The precipitated virus was eluated with phosphate buffer (of pH 7.

[Development of a method for the quantitative determination of the immunizing antigen (140S) of the foot-and-mouth disease virus].

Attempts were made to work out a method for measuring the amount of the 140 S antigen in virus suspensions. Early postinfection sera were obtained from guinea pigs against the productional strains of the foot-and-mouth disease virus which were used in the radial immunodiffusion test. The investigated virus suspensions were concentrated 50 to 200 times and were placed in a CsCl gradient for gradient centrifugation.