Tissue-Engineered Oral Epithelium for Dental Material Testing: Toward Biomimetic Models.

Tissue-engineered oral epithelium (ΤΕΟΕ) was developed after comparing various culture conditions, including submerged (SUB) and air-liquid interface (ALI) human cell expansion options. Barrier formation was evaluated via transepithelial electrical resistance (TEER) and calcein permeation via spectrofluorometry. TEOE was further assessed for long-term viability via live/dead staining and development of intercellular connections via transmission electron microscopy.

Managing Temporomandibular Joint Osteoarthritis by Dental Stem Cell Secretome.

The potential therapeutic role of the Dental Pulp Stem Cells Secretome (SECR) in a rat model of experimentally induced Temporomandibular Joint (TMJ) Osteoarthritis (OA) was evaluated. Proteomic profiling of the human SECR under specific oxygen tension (5% O2) and stimulation with Tumor Necrosis Factor-alpha (TNF-α) was performed. SECR and respective cell lysates (CL) samples were collected and subjected to SDS-PAGE, followed by LC-MS/MS analysis.

Effectiveness of conservative therapeutic modalities for temporomandibular disorders-related pain: a systematic review.

Purpose: The aim of this systematic review was to evaluate the effectiveness of conservative different therapeutic modalities for temporomandibular disorders (TMD) pain.

Materials And Methods: An electronic systematic search was conducted in the MEDLINE (PubMed) database to identify the randomized clinical trials (RCTs) published between 2001 and 2021. The following, simple or multiple conjunctions, search keywords were selected: TMD pain, TMD management or conservative treatment or treatment strategies and TMD pain, therapeutic modalities or interventions and TMD.

Effect of different zirconia surface pretreatments on the flexural strength of veneered Y-TZP ceramic before and after in vitro aging.

Purpose: The investigation of zirconia core surface pretreatments on the flexural strength of bilayered zirconia ceramics before and after artificial accelerating aging.

Methods: Ninety bar-shaped specimens were manufactured from Yttria Stabilized Tetragonal Zirconia Polycrystal (Y-TZP) and divided in three groups depending on zirconia surface pretreatment before veneering: layering with liner, pretreatment with silane-containing gas flame (SGF) with the Silano-Pen device and alumina air-abrasion. Half of the veneered specimens in each group (n=15) underwent artificial accelerating aging.

Screwmentable implant-supported prostheses: A systematic review.

Statement Of Problem: Screwmentable prostheses were developed to combine the benefits of screw retention and cement retention. However, data are limited on the clinical performance of this type of prosthesis.

Purpose: The purpose of this systematic review was to collect scientific evidence on screwmentable prostheses and evaluate their long-term clinical behavior.