A preliminary comparative study of a dipstick colloidal dye immunoassay and two antigen-detection ELISAs for diagnosis of Trypanosoma evansi infection in cattle.

A dipstick colloidal dye immunoassay (DIA) was developed for the field diagnosis of Trypanosoma evansi infection using affinity-purified polyclonal antibodies (PcAbs) and the monoclonal antibody (McAb) 8B9. PcAbs were adsorbed onto Palanil Red dye particles and used as dye reagents. Dipsticks were dotted with four different antibodies; normal rabbit and mouse IgGs as negative controls, and anti-T.

Seroprevalence of antibodies to Neospora caninum and associated abortion in dairy cattle from central Thailand.

A total of 904 sera from dairy cattle in 11 provinces of central Thailand were tested for antibodies to Neospora caninum employing the indirect fluorescent antibody test (IFAT). Fifty four (6%) cattle were positive in IFAT, titres of 1:200 (16 cattle), 1:400 (9 cattle), 1:800 (14 cattle), 1:1600 (7 cattle), 1:3200 (6 cattle) and two positives. No significant difference was observed among the provinces.