Publications by authors named "P Spangenberg"
Infection with enterohemorrhagic E. coli (EHEC) causes severe changes in the brain leading to angiopathy, encephalopathy and microglial activation. In this study, we investigated the role of tumour necrosis factor alpha (TNF-α) for microglial activation and brain pathology using a preclinical mouse model of EHEC infection.
View Article and Find Full Text PDFMultimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms by mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing software solutions for MALDI MSI data analysis are incomplete, require programming skills and contain laborious manual steps, hindering broadly applicable, reproducible, and high-throughput analysis to generate impactful biological discoveries. Here, we present msiFlow, an accessible open-source, platform-independent and vendor-neutral software for end-to-end, high-throughput, transparent and reproducible analysis of multimodal imaging data.
View Article and Find Full Text PDFBackground: The tumour microenvironment significantly influences the clinical response of patients to therapeutic immune checkpoint inhibition (ICI), but a comprehensive understanding of the underlying immune-regulatory proteome is still lacking.
Objectives: To decipher targetable biologic processes that determine tumour-infiltrating lymphocytes (TiLs) as a cellular equivalent of clinical response to ICI.
Methods: We mapped the spatial distribution of proteins in TiL-enriched vs.
Article Synopsis
- Recent advances in light-sheet microscopy now allow for 3D analysis of microvascular networks in brain tissue without the need for histological sectioning, which was previously a barrier.
- In a study involving mice after a stroke model, researchers observed significant changes in microvessels over time, including an initial loss followed by a reemergence and increased complexity of small microvessels within 56 days.
- The administration of FTY720 (Fingolimod) after ischemia enhanced the growth and branching of small microvessels, highlighting its potential in improving microvascular architecture following a stroke.
Article Synopsis
- The study investigates the effects of thyroid hormones (TH) on cardiac function, specifically focusing on which thyroid hormone receptors (TR) mediate these effects and through which mechanisms.
- Researchers compared mice with knockout versions of TRα and TRβ receptors to wild-type mice and assessed heart changes after treatment with T3, a form of thyroid hormone.
- Results showed that T3-induced ventricular hypertrophy primarily involved noncanonical TRα action, while heart rate regulation was mainly governed by canonical TRα action, with TRβ being able to compensate for some TRα functions.