Publications by authors named "P Situ"
Purpose: To investigate the roles of neural adaptation and sensitization in contact lens discomfort (CLD).
Methods: Cooling stimuli (20 °C) were applied to the cornea in a group comprising 24 symptomatic and 25 asymptomatic contact lens (CL) wearers as well as 15 non-CL wearing controls, using a computerized Belmonte esthesiometer. The adaptation paradigm consisted of 20 repetitive stimuli at threshold, sub- and supra-threshold levels.
Hepatocytes exhibit a multi-polarized state under the in vivo physiological environment, however, human embryonic stem cell-derived hepatocytes (hEHs) rarely exhibit polarity features in a two-dimensional (2D) condition. Thus, we hypothesized whether the polarized differentiation might enhance the maturity and liver function of hEHs. In this study, we obtained the polarized hEHs (phEHs) by using 2D differentiation in conjunct with employing transwell-based polarized culture.
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- Human pluripotent stem cells (hPSCs) are crucial for regenerative medicine, but producing them at scale in 3D suspension cultures has been a challenge; dextran sulfate (DS) has shown promise in preventing excessive cell adhesion and large aggregate formation.
- The study investigated the effects of DS on cellular adhesion molecules (CAMs) in hPSCs, revealing that DS treatment significantly down-regulated E-cadherin and intercellular adhesion molecule 1 (ICAM1), which are key players in hPSC adhesion.
- Additionally, the research indicated that DS not only inhibited hPSC aggregation but also activated Wnt signaling pathways by up-regulating related genes, thereby influencing the expression of crucial CAMs involved in
Purpose: To explore the effect of time on grading corneal fluorescein and conjunctival lissamine green staining in dry eye disease (DED).
Methods: Photographs of 68 subjects with non-Sjogren's DED (nSS DED) and 32 with Sjogren's DED (SS DED) were taken of corneal fluorescein staining, then conjunctival lissamine green staining every 30 s for at least 5 min. Photographs of one randomly selected eye were then randomly ordered and graded on a scale from 0 to 5 (severe staining) by two clinicians, masked to both site and subject.
Purpose: In this study, we apply psychophysical scaling principles based on physical (photometric) attributes of images to better understand the factors involved in clinician judgement of ocular surface staining and, using that knowledge, to develop photographic scales for the assessment of staining for dry eye (DE) and related conditions.
Methods: Subjects with noninfectious ocular surface staining were enrolled at five clinical sites. Following instillation of fluorescein, photographs of corneal staining were taken every 30 seconds for at least 5 minutes.