Publications by authors named "P Serhal"
The human blastocyst contains the pluripotent epiblast from which human embryonic stem cells (hESCs) can be derived. ACTIVIN/NODAL signaling maintains expression of the transcription factor NANOG and in vitro propagation of hESCs. It is unknown whether this reflects a functional requirement for epiblast development in human embryos.
View Article and Find Full Text PDFDuring the first week of development, human embryos form a blastocyst composed of an inner cell mass and trophectoderm (TE) cells, the latter of which are progenitors of placental trophoblast. Here, we investigated the expression of transcripts in the human TE from early to late blastocyst stages. We identified enrichment of the transcription factors GATA2, GATA3, TFAP2C and KLF5 and characterised their protein expression dynamics across TE development.
View Article and Find Full Text PDFImpact of double trophectoderm biopsy on reproductive outcomes following single euploid blastocyst transfer.
Eur J Obstet Gynecol Reprod Biol
July 2024
Objectives: To study the effect of double trophectoderm biopsy on clinical outcomes following single euploid blastocyst transfer.
Study Design: Retrospective cohort study of 2046 single euploid frozen-thawed blastocyst transfers from January 2015 to June 2022 in a single centre. All patients undergoing a frozen-thawed embryo transfer (FTET) cycle with euploid blastocysts, biopsied for any indication, were included.
Article Synopsis
- Fibrodysplasia ossificans progressiva (FOP) is a rare genetic condition causing disabilities due to abnormal bone formation, with no known effective treatments and an incidence of about 1 in 2 million people.
- A 30-year-old woman with FOP utilized in vitro fertilization (IVF) and preimplantation genetic testing for monogenic disorder (PGT-M) to conceive a healthy baby unaffected by the condition.
- After a complex IVF process involving medication to reduce complications, the couple successfully had a baby through a third embryo transfer, resulting in a healthy birth at 37 weeks, with the child unaffected by FOP.
Purpose: Utilising non-invasive imaging parameters to assess human oocyte fertilisation, development and implantation; and their influence on transcriptomic profiles.
Methods: A ranking tool was designed using imaging data from 957 metaphase II stage oocytes retrieved from 102 patients undergoing ART. Hoffman modulation contrast microscopy was conducted with an Olympus IX53 microscope.