Acid base status during treatment of chronic uremia with diafiltration.

Treatment of chronic uremia by hemodiafiltration requires replacement of the filtrate. Using Ringer's solution alone, there is a depression of pH because of bicarbonate loss. To bring the acid base status back to normal, sodium lactate in increasing concentrations (283 mg% = 32 mM/1, 361 mg% = 40 mM/1; 462 mg% = 51 mM/1; 508 mg% = 57 mM/1) was added to the replacement fluid.

[A modification of Laurell's one-dimension electrophoresis using polyvalent antisera (author's transl)].

A modification of the Laurell method is described which makes it possible to measure very small amounts of proteins. The use of the intermediate gel technique allows the simultaneous determination of two or more proteins by one-dimension electrophoresis. The method is useful for differentiation of organ-specific proteins from serum proteins, for identification of identical and not-identical antibodies in antisera and for checking protein products and antisera for their purity.