Cloning of ligand-specific cell lines via gene transfer: identification of a D2 dopamine receptor subtype.

Using rat genomic DNA, we have established a transfected mouse fibroblast cell line that expresses a spiperone binding site with the pharmacological characteristics of a D2 dopamine receptor. The expressed D2 receptors are the product of a gene that is distinct from that reported by Bunzow et al. [Bunzow, J.

Isolation of NILE glycoprotein-related cDNA probes.

The nerve growth factor (NGF)-induced large external (NILE) glycoprotein is an NGF-inducible surface component of PC12 cells that is also widely distributed in the nervous system. It has recently been shown to be indistinguishable from the high-molecular-weight species of the brain antigens L1 and neuron-glia cell adhesion molecule (Ng-CAM) and may have a function in regulating cell adhesion in the developing nervous system. We have used polyclonal anti-NILE antisera to screen a lambda gt11 cDNA library made from NGF-treated PC12 cells.

Probable identity of NILE glycoprotein and the high-molecular-weight component of L1 antigen.

We report here that anti-L1 antiserum, raised against material from embryonic brain, and anti-NILE antiserum, raised against purified NILE (nerve growth factor-inducible large external) glycoprotein of PC12 cells, immunoprecipitate from PC12 cells material of the same apparent molecular weight (230 kilodaltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Furthermore, each of these immune reagents has the capacity to clear from a PC12 cell extract all of the 230-kilodalton antigen recognized by the other antiserum. Finally, in immunohistochemical staining of developing cerebellum the two antisera exhibit very similar staining patterns.

Selective staining of large projection neurons by monoclonal antibody to a glycoprotein of PC12 cells.

A monoclonal antibody has been produced against glycoproteins prepared from PC12 cells. This antibody stains PC12 cells in a rimlike fashion, whether they are fixed or living. When neuronally differentiated PC12 cells are used, neurites as well as somata stain.

Inhibitory mechanism in zebrafish optic tectum: visual response properties of tectal cells altered by picrotoxin and bicuculline.

In previous work we described 4 types of visual response among tectal cells of the zebrafish. Cells of one class, type I, have no spontaneous activity, but respond phasically at ON and OFF. Their responses to moving edges, to stimuli that grow in size, and to stimuli equal in size and shape to the whole receptive field (RF) suggest that these cells may receive inhibitory input from near neighbor cells of the same type in the tectum, as well as excitatory input from retinal fibers.