Publications by authors named "P S Schoenmakers"
Multidimensional chromatography offers enhanced chromatographic resolution and peak capacity, which are crucial for analyzing complex samples. This study presents a novel comprehensive online multidimensional chromatography method for the lipidomic analysis of biological samples, combining lipid class and lipid species separation approaches. The method combines optimized reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC) in the first dimension, utilizing a 150 mm long C18 column, with ultrahigh-performance supercritical fluid chromatography (UHPSFC) in the second dimension, using a 10 mm long silica column, both with sub-2 μm particles.
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- A new method combines photocatalytic hydrogen generation with green enzymatic reductions, showing a significant increase in efficiency.
- The system, using Pt(x)@TiO, produces hydrogen at levels much higher than existing technologies, based on a unique photocatalytic mechanism rather than thermal reactions.
- The approach successfully synthesizes valuable compounds at low pressure and room temperature, indicating potential applications in flavors, fragrances, and pharmaceuticals.
Article Synopsis
- Polyesters are important in high-end industrial applications, but their complex chemical properties make them challenging to analyze effectively.
- A new analytical method combining normal-phase liquid chromatography, size-exclusion chromatography, ultraviolet-light spectroscopy, and high-resolution mass spectrometry allows for detailed characterization of polyesters, focusing on their end-group functionality and molecular weight distributions.
- This method enables comprehensive analysis of polyesters, providing crucial information on their chemical composition and properties that cannot be attained through traditional techniques, making it highly valuable for industrial applications.
End groups of poly(Lactide-co-glycolide) (PLGA) play an important role in determining the properties of polymers for use in drug delivery systems. For instance, it has been reported that the encapsulation efficiency in PLGA microspheres varies significantly between ester-terminated and acid-terminated PLGA. More importantly, the in-vivo degradation time of such polymer excipients is influenced by the functional end-group of the copolymer used.
View Article and Find Full Text PDFThe characterization of proteins and complexes in biological systems is essential to establish their critical properties and to understand their unique functions in a plethora of bioprocesses. However, it is highly difficult to analyze low levels of intact proteins in their native states (especially those exceeding 30 kDa) with liquid chromatography (LC)-mass spectrometry (MS). Herein, we describe for the first time the use of nanoflow ion-exchange chromatography directly coupled with native MS to resolve mixtures of intact proteins.
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