Inhibition of pyruvate dehydrogenase complex by moniliformin.

The mechanism for the inhibition of pyruvate dehydrogenase complex from bovine heart by moniliformin was investigated. Thiamin pyrophosphate proved to be necessary for the inhibitory action of moniliformin. The inhibition reaction was shown to be time-dependent and to follow first-order and saturation kinetics.

Inhibition of esophageal carcinogenesis in corn-fed rats by riboflavin, nicotinic acid, selenium, molybdenum, zinc, and magnesium.

The effect of individual vitamins and minerals on esophageal carcinogenesis was assessed by supplementing a diet (based on corn) that was marginally deficient in many micronutrients. The test diets were fed to weanling inbred BD IX rats for 45 days; they were then given five subcutaneous doses (3 mg/kg body wt) N-nitrosomethylbenzylamine (MBN) over two and one-half weeks. The diets were continued until termination: 150 days later; at that time all rats were still clinically normal.