Publications by authors named "P Rye"
Article Synopsis
- * This study investigates the enhancement of mucus penetration in cationic siRNA and mRNA RTNs by using low molecular weight alginate oligosaccharides (OligoG and OligoM), finding that they significantly improve diffusion rates, especially OligoG.
- * Results show that while RTNs maintain their structure after passing through mucus and OligoM-coated mRNA RTNs are most efficient in transfection, the combination of RTNs with alginate ol
Oligosaccharides from uronic acid-containing polysaccharides can be produced either by chemical or enzymatic degradation. The benefit of using enzymes, called lyases, is their high specificity for various glycosidic linkages. Lyases cleave the polysaccharide chain by an β-elimination reaction, yielding oligosaccharides with an unsaturated sugar (4-deoxy-l-erythro-hex-4-enepyranosyluronate) at the non-reducing end.
View Article and Find Full Text PDFObjective: The speech intelligibility index (SII) is used to quantify the audibility of the speech. This study examined the relationship between self-reported hearing aid (HA) outcomes and the difference in aided SII (SII) calculated from the initial fit (IF) gain and that prescribed as per the second generation of National Acoustic Laboratory Non-Linear (NAL-NL2).
Design: A prospective observational study.
Low molecular weight alginate oligosaccharides have been shown to exhibit anti-microbial activity against a range of multi-drug resistant bacteria, including . Previous studies suggested that the disruption of calcium (Ca)-DNA binding within bacterial biofilms and dysregulation of quorum sensing (QS) were key factors in these observed effects. To further investigate the contribution of Ca binding, G-block (OligoG) and M-block alginate oligosaccharides (OligoM) with comparable average size DPn 19 but contrasting Ca binding properties were prepared.
View Article and Find Full Text PDFAlginate oligosaccharides enhance the antifungal activity of nystatin against candidal biofilms.
Front Cell Infect Microbiol
February 2023
Background: The increasing prevalence of invasive fungal infections in immuno-compromised patients is a considerable cause of morbidity and mortality. With the rapid emergence of antifungal resistance and an inadequate pipeline of new therapies, novel treatment strategies are now urgently required.
Methods: The antifungal activity of the alginate oligosaccharide OligoG in conjunction with nystatin was tested against a range of spp.