One hundred-year-old human corneal and transplanted corneal graft endothelium.

Purpose: To investigate and analyse one hundred-year-old endothelial cells of human transplanted corneal grafts and compare them with intact endothelium of unoperated eyes.

Subjects And Methods: Specular microscopy was performed on seven individuals (14 eyes) aged >100 years and on seven transplanted corneas with endothelial cells of at least 100 years old. All keratoplasties were performed by the same surgeon (PR) 25-35 years before the time of examination.

Follow-up study of human corneal endothelial cells, photographed in vivo before enucleation and 20 years later in grafts.

Purpose: To evaluate the long-term (20-year) survival of transplanted human corneal endothelial cells.

Methods: The donor endothelium had been photographed 20 years ago with a specular microscope both before enucleation in the melanomatous eye and in situ after keratoplasty. The same donor endothelial cells were now photographed again 20 years later with a modern specular microscope to ascertain the morphology and cell density of the grafts.

Effects of experimental exposure to triethylamine on vision and the eye.

Objectives: To determine the effect of triethylamine (TEA) on the cornea and to evaluate the cause of blurred vision. To find the lowest observed effect concentration of exposure to TEA.

Methods: Four people were exposed to TEA for 4 hours at concentrations of 40.

Effects of long-term contact lens wear on the corneal endothelium.

Purpose: To analyze the long-term effects of soft and hard (PMMA) contact lenses (CL) on the human corneal endothelium.

Methods: One hundred and one contact lens wearers with > or =10 years' wearing time and 50 healthy control subjects were examined with specular microscope.

Results: The mean corneal endothelial cell densities of the CL wearers (2846 cells/mm2) and of the control eyes (2940 cells/mm2) differed by 94 cells/mm2 (p<0.

Photoastigmatic keratectomy for correction of astigmatism in corneal grafts.

Background: The efficacy and safety of excimer laser photoastigmatic refractive keratectomy (PARK) for treatment of astigmatism after penetrating keratoplasty (PKP) was evaluated in this study.

Methods: A VisX 20/20 excimer laser was used to correct the regular astigmatic component of the grafts. The epithelium was removed manually in seven cases and in three patients with the PTK mode of the laser.