Publications by authors named "P Rogowsky"
Meeting the challenges of agroecological transition in a context of climate change requires the use of various strategies such as biological regulations, adapted animal and plant genotypes, diversified production systems, and digital technologies. Seeds and plants, through plant breeding, play a crucial role in driving these changes. The emergence of genome editing presents a new opportunity in plant breeding practices.
View Article and Find Full Text PDFThe growing world population and global increases in the standard of living both result in an increasing demand for food, feed and other plant-derived products. In the coming years, plant-based research will be among the major drivers ensuring food security and the expansion of the bio-based economy. Crop productivity is determined by several factors, including the available physical and agricultural resources, crop management, and the resource use efficiency, quality and intrinsic yield potential of the chosen crop.
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- Grasses belong to a monocotyledonous family, which includes important crops like wheat, rice, and barley, stemming from a common ancestor around 100 million years ago.
- The study analyzes genomic data from ten grass species to understand how whole genome duplications (WGD) affect genome changes and adaptations in grasses, revealing that while lineages with a shared WGD show similar structural changes, these patterns can't be generalized across all WGD events due to varying factors like selection and crop domestication.
- The research highlights that while polyploidy is crucial for the evolutionary success of grasses, its specific effects on different lineages are complex and require a broader, comparative approach for deeper insight.
Introduction: Despite its rapid worldwide adoption as an efficient mutagenesis tool, plant genome editing remains a labor-intensive process requiring often several months of culture to obtain mutant plantlets. To avoid a waste in time and money and to test, in only a few days, the efficiency of molecular constructs or novel Cas9 variants (clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9) prior to stable transformation, rapid analysis tools are helpful.
Methods: To this end, a streamlined maize protoplast system for transient expression of CRISPR/Cas9 tools coupled to NGS (next generation sequencing) analysis and a novel bioinformatics pipeline was established.