Publications by authors named "P Rerkyen"
Background: In the gingival sulcus, effective and balanced innate and adaptive immune responses against subgingival plaque microbiome are crucial to maintain immune homeostasis. In this study, we investigated the memory T cell subsets in healthy gingiva and periodontitis tissues.
Methods: Anatomical localization of T cells (CD3 , CD4 , and CD8 ) in healthy gingiva and periodontitis tissues were examined immunohistochemically.
Article Synopsis
- The study analyzed human tissue biopsies from healthy gums, gingivitis, and periodontitis to examine the types of B cells present in different periodontal conditions.
- The findings revealed that during periodontitis, plasma cells (CD19(+)CD27(+)CD38(+)CD138(+)HLA-DR(low)) were the predominant B cell type, particularly located at the base of periodontal pockets, unlike in healthy tissue where memory B cells were more common.
- Pro-inflammatory molecules that promote B cell activity were found in higher levels in gingivitis and periodontitis tissues, indicating that these conditions foster an immune response largely directed against periodontal pathogens.
Objective: Observational evidence suggests association between periodontitis and atherosclerotic vascular disease (ASVD), however the cause-effect remains unclear. In this study, we investigated the mechanistic link of the two diseases by measuring production of interleukin (IL)-1β, a potent inflammatory cytokine, induced via inflammasome activation by a key periodontal pathogen--Porphyromonas gingivalis LPS and cholesterol crystals (CC).
Methods: An in vitro model of primary human monocyte-derived macrophages (M1 and M2 macrophages) and coronary artery endothelial cells (HCAEC) was employed as a source of inflammasome product-IL-1β.
Although periodontal tissue is continually challenged by microbial plaque, it is generally maintained in a healthy state. To understand the basis for this, we investigated innate antiviral immunity in human periodontal tissue. The expression of mRNA encoding different antiviral proteins, myxovirus resistance A (MxA), protein kinase R (PKR), oligoadenylate synthetase (OAS), and secretory leukocyte protease inhibitor (SLPI) were detected in both healthy tissue and that with periodontitis.
View Article and Find Full Text PDFObjective: Aggregatibacter actinomycetemcomitans is known to be a major cause of localized aggressive periodontitis. Previous research has suggested that A. actinomycetemcomitans can damage many types of host cells.
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