Microsensor in Microbioreactors: Full Bioprocess Characterization in a Novel Capillary-Wave Microbioreactor.

Microbioreactors (MBRs) with a volume below 1 mL are promising alternatives to established cultivation platforms such as shake flasks, lab-scale bioreactors and microtiter plates. Their main advantages are simple automatization and parallelization and the saving of expensive media components and test substances. These advantages are particularly pronounced in small-scale MBRs with a volume below 10 µL.

Dose-response effect of in vivo administration of endotoxin on polymorphonuclear leukocytes oxidative burst.

In vitro, endotoxin primes polymorphonuclear leukocytes (PMNs) to respond with a greater oxidative burst. The purpose of the present study was to investigate the in vivo effect of a wide range of single endotoxin bolus doses using a rat model. PMNs were subsequently challenged in vitro with phorbol ester to produce reactive oxygen intermediates (ROI).

Dialysable nonspecific inhibitor of lymphocyte proliferation related to E-receptors (CD2).

Several investigators have pointed out that lymphocytic function may be profoundly affected by soluble factors occurring in human serum. It was observed, previously, that the addition of normal human serum dialysate (NHSD), at the beginning of lymphocyte cultures, inhibited the proliferative response to phytohemagglutinin (PHA) and to allogeneic cells. This suppressive effect was removed by previous absorption of NHSD with sheep erythrocytes (E).