CD19-CAR T-cell therapy induces deep tissue depletion of B cells.

Objectives: CD19-targeting chimeric antigen receptor (CAR) T-cell therapy can induce long-term drug-free remission in patients with autoimmune diseases (AIDs). The efficacy of CD19-CAR T-cell therapy is presumably based on deep tissue depletion of B cells; however, such effect has not been proven in humans in vivo.

Methods: Sequential ultrasound-guided inguinal lymph node biopsies were performed at baseline and after CD19-CAR T-cell therapy in patients with AIDs.

Prevalence of molar incisor hypomineralization and demands for treatment according to the severity of its clinical manifestation.

Aim: Assess the prevalence and severity of molar incisor hypomineralization (MIH) in children 6- to 12-year-old and correlate MIH severity with the complexity of treatment demands.

Materials And Methods: Between March and October 2023, 2,136 children were screened for MIH by two calibrated examiners. MIH severity was categorized in mild, moderate, severe, and very severe.

Effects of ionizing radiation on osteoblastic cells: In vitro insights into the etiopathogenesis of osteoradionecrosis.

Objective: This in vitro study aimed to analyze the effects of ionizing radiation on immortalized human osteoblast-like cells (SaOS-2) and further assess their cellular response in co-culture with fibroblasts. These analyses, conducted in both monoculture and co-culture, are based on two theoretical models of osteoradionecrosis - the theory of hypoxia and cellular necrosis and the theory of the radiation-induced fibroatrophic process.

Design: SaOS-2 cells were exposed to ionizing radiation and evaluated for cell viability, nitric oxide (NO) production, cellular morphology, wound healing, and gene expression related to the PI3K-AKT-mTOR pathway.

Nurses' perspectives on the use of telemonitoring in the management of people with diabetes and hypertension.

Objectives: to understand the perspective of nurses on the use of telemonitoring in the management of people with type 2 diabetes mellitus and arterial hypertension in primary care.

Methods: this qualitative research involved sixteen nurses from eight municipalities in Paraná. Data were collected between November 2022 and January 2023 through inperson or remote interviews, which were audio-recorded and subjected to content analysis.