2,5-Di-t-amyl-p-benzoquinone: an antioxidant encountered during the analysis of human tissues.

The title compound was found in extracts of several human tissues, including neurological tumors, adult brain and fetal brain. It was also present in adhesive tapes used in the laboratory to label glassware, but not in sufficient quantities to be responsible for contamination of the tissues. No source of contamination was found in the hospital.

A method for enumeration ot T and B lymphocytes in tissues.

A method for the identification of T and B lymphocytes in tissue specimens is described. A sonication technique results in viable relatively pure lymphocyte populations which are easily classified by their surface markers. This readily reproducible method can become a standard laboratory procedure in the evaluation of disease states which require such information related to the classification of lymphocyte cell origin.

Horseradish peroxidase labeled antisera--a diagnostic method for evaluating the percentages of B lymphocytes in peripheral blood.

Horseradish peroxidase labeled antisera supplied commercially was used to evaluate B lymphocytes in peripheral blood. This technique not only gave comparable results to the conventional fluorescein method, but also proved to be more advantageous.

Effect of lid suture on retinal ganglion cells in Macaca mulatta.

Previous work has established the pronounced effect of unilateral lid suture during visual immaturity on cell growth in the lateral geniculate nucleus of cats, dogs and monkeys. Most investigators have reported the retinas to be grossly normal but only a few quantitative studies are available and the results are contradictory. We have compared cell section diameters and cell density of parafoveal retinal ganglion cells in the deprived and non-deprived eye of Macaca mulatta after the lids of one eye were studied between the ages of 1-9 weeks for varying periods of time.

The effects of monocular visual deprivation: disuse or binocular interaction?

Patches of transnueronal degeneration were produced in the lateral geniculate nucleus (LGN) by retinal laser burns and a tarsorrhaphy was performed in the contralateral eye of Macaca mulatta during infancy. The adverse effect of unilateral visual deprivation on geniculate cell growth was absent in those portions of deprived LGN laminae that were located opposite a degenerated patch. This finding supports the hypothesis that arrest of geniculate cell growth from unilateral visual deprivation is caused by abnormal binocular interaction.