Comparison of lateral flow assay and polymerase chain reaction for diagnosis of canine distemper.

In present study, 50 nasal and conjunctival pooled swab samples from 48 dogs, one each from leopard and cat were collected, which were suspected for canine distemper from Anand and Ahmedabad districts during period of January-21 to June-21. Out of the 50 samples, 18% were positive for CD by Lateral flow assay (LFA). Using LFA, one each sample from leopard and cat samples were found negative for CD by LFA.

Fibronectin dependent upregulation of matrix metalloproteinases in hepatic stellate cells.

Activation and transition of hepatic stellate cells (HSCs) to myofibroblast (MFB)-like cells is influenced by growth factors, cytokines and matrix proteins like fibronectin (FN). To examine whether the FN-dependent transition of HSCs is mediated through FN receptor, a marker function, such as matrix metallo-proteinase (MMP) production by HSCs in primary culture was studied. An upregulation of MMP production by HSCs maintained on FN was observed.