Comparison of embryonic competence and clinical outcomes between early and late cumulus cell removal for in vitro fertilization.

Objective: The impact of early mechanical removal of cumulus cells on fertilization and embryonic development is not yet precisely known. This study aimed to investigate the effects of early and late cumulus cell removal on fertilization, polyspermy, embryonic development potential, blastocyst development, and clinical outcomes.

Methods: A prospective study was conducted of patients who underwent in vitro fertilization between September 2019 and October 2020.

The effect of insemination methods on in vitro maturation outcomes.

Objective: The aim of this study was to compare the effects of conventional insemination (in vitro fertilization [IVF]) and intracytoplasmic sperm injection (ICSI) on the fertilization, developmental competence, implantation potential, and clinical pregnancy rate of embryos derived from in vitro matured oocytes of patients with polycystic ovary syndrome (PCOS).

Methods: A prospective study was carried out among 38 PCOS patients who had undergone in vitro maturation (IVM) treatment. In total, 828 immature oocytes were collected from 42 cycles and randomly assigned for insemination by IVF (416 oocytes) or ICSI (412 oocytes).

Developmental competence of -matured human oocytes obtained from pregnant and non-pregnant women.

Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of -matured human oocytes derived from pregnant and non-pregnant women.

Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later.

Comparison of blastocyst and Sage media for in vitro maturation of human immature oocytes.

In vitro maturation (IVM) of human oocytes is an attractive alternative to conventional assisted reproductive technology (ART) treatment, as it involves no or minimal ovarian stimulation. Currently, commercialized media specifically designed for IVM are often used. These media are expensive, have limited shelf life, and must be ordered in advance.

Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section.

Aim: To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section.

Methods: Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to participate in the study. Retrieved oocytes were cultured in blastocyst medium supplemented with 75 IU/L of human menopausal gonadotropin.