[Chlamydia trachomatis infection of the genitals in women].

Direct and by immunofluorescence examination of smears, conducted on 682 women with different diseases of the cervix uteri, allowed the detection of Chl. trachomatis in 10.5% and 11.

Investigations on the role of some viral, chlamydian, rickettsian and mycoplasmic agents in several gynecological diseases.

This work presents the results of a study on some viral, chlamydial, rickettsial and mycoplasmal agents incidence and their role on several gynecological ailments: vulvovaginitis, cervicitis, cervicovaginitis. The presence of some viral, chlamydial, rickettsial and mycoplasmal antigens in the vaginal secretion was investigated by immunofluorescence technique, isolation on selective media for Mycoplasma, and MGG staining in a group of 60 women with gynecopathies (group A) and in a control group (also 60 clinically healthy women) (group B). This study pointed out to higher incidence of chlamydial and mycoplasma antigens as well as of parainfluenza virus types I and III, adenovirus and herpes virus type II in group A.

Viral and rickettsial etiology of an outbreak of acute respiratory tract infection.

An outbreak of 111 cases of acute respiratory tract infection was recorded in a community of the town "T" in April-May 1984. The clinical picture was severer than usual; 28% of the cases had to be hospitalized, average absenteeism being as high as 26 days per case. Serological investigations demonstrated the previous circulation of influenza virus B/Singapore/222/79 and the simultaneous circulation during the outbreak of influenza virus A/England/333/80 (H1N1) and of Rickettsia burneti (as also ascertained by isolation in the chick embryo of the former and by visualization by immunofluorescence in exfoliated cells of the latter pathogen).

Contribution of the rapid viral diagnosis by immunofluorescence to the choice of therapy in uveitis cases.

Viral and inframicrobial antigens were detected by immunofluorescence (IF) in exfoliated conjunctival and corneal cells from 22 patients with uveitis, 2-9 different antigens being simultaneously visualized in 17 of the cases. The treatment applied on the ground of the etiological diagnosis specified by IF reactions consisted in the administration of tetracycline, Flumidin (Virustat), standard gamma-globulin or specific antiherpes immunoglobulin, the NIVGRIP inactivated influenza vaccine. This therapy led to persistent recovery (coincident with the disappearance of the antigens from conjunctival cells) in 15 cases and to clinical improvement in 7 cases.

Rapid detection by immunofluorescence of multiple viral infections in patients with keratitis.

The presence of 12 viral (herpes 1 and 2, influenza A(H1N1), A(H3N2) and B, parainfluenza 1, 2, 3, adenovirus 5) and inframicrobial (Chlamydia, mycoplasma, Rickettsia burneti) antigens was investigated by direct or indirect immunofluorescence (IF) reactions in exfoliated conjunctival cells from 110 patients with nonbacterial keratitis and keratoconjunctivitis. A rapid etiological diagnosis could be obtained in 101 (92%) of the cases, parainfluenza and herpes antigens being the most frequently detected. In most of the cases the simultaneous presence of several antigens was made evident.