Postganglionic, direct axo-axonal contacts on the splenic nerve.

The splenic nerve is made up almost exclusively of adrenergic fibers. Consequently it was used as a model system in the study of autonomic sympathetic neurotransmission. The splenic nerve regulates the vasoconstriction and volume reduction of the spleen.

Secretory vesicle-specific antibodies in the confocal study of exo-endocytosis dynamics.

The study of secretory vesicle dynamics is a continuing challenge. Classically it was studied using biochemical techniques, such as subcellular fractionation and immunoprecipitation, combined with time-consuming electron microscopy studies. The recent development of confocal microscopy, giving in-focus optical section images throughout the thickness of a fluorescently labeled sample, allows scientists to study the key events in the secretory cycle at the level of light microscopy.

The storage of noradrenaline, neuropeptide Y and chromogranins in and stoichiometric release from large dense cored vesicles of the undifferentiated human neuroblastoma cell line SH-SY5Y.

Sucrose gradient centrifugation combined with electron microscopy revealed that undifferentiated SH-SY5Y cells contain predominantly one population of noradrenaline containing vesicles, i.e. large dense cored vesicles.

Differential ultrastructural distribution of synapsin and synaptophysin proximal to a ligation in bovine splenic nerve.

Synaptophysin and synapsin, closely correlated on synaptic vesicles in terminals, may show a differential distribution at synapse formation and maturation. In order to disclose the fine structural details of these differences, synapsin and synaptophysin distribution was studied by immunocytochemistry on ligated bovine splenic axons in vitro and compared with terminals in the vas deferens. In the synaptic differentiations taking place proximally synapsin could only be detected on the accumulating elements of the axonal reticulum.