Continuous free flow electrophoresis for preparative chiral separations of piperoxan using sulfated beta-cyclodextrin.

Continuous free flow electrophoresis was investigated as a tool for the preparative chiral separation of piperoxan using a sulfated cyclodextrin chiral additive. In the absence of chiral additive, the sample stream was deflected cathodically. However, the presence of sulfated cyclodextrin in the run buffer caused anodic deflection and splitting of the sample stream into two streams, each enriched in one enantiomer.

A new method of scaling up free flow electrophoresis.

Free flow electrophoresis (FFE) has been utilized for the separation of proteins and cells for many years and has evolved into the most promising method of continuous separation of biomolecules. One of the major drawbacks inherent in FFE in the past, however, is the thermal convection caused by joule heating which occurs whenever a current is applied across a conducting liquid medium. To provide efficient heat dissipation, the cross-section of traditional FFE units is restricted to approximately 1 mm, which limits sample throughput.

Purification of erucic acid by preparative high-performance liquid chromatography and crystallization.

Erucic acid (C22:l fatty acid) has been found to be useful in the treatment of adrenoleukodystrophy (ALD). It appears to work by reducing the blood levels of very-long-chain fatty acids (VLCFAs) which destroy the myelin sheaths of the nerves. Erucic acid was purified by reversed-phase high-performance liquid chromatography (HPLC) on columns packed with YMC C18 (10-20 microns, 120 A).