Involving People With Lived Experience in Electronic Health Record Database Studies Reflections and Learning From the CHOOSE Study.

Background: Patient and public involvement and engagement (PPIE) is integral to health research. Reporting of PPIE methods and impact is becoming increasingly common in health research. However, reporting on PPIE in studies using large, routinely collected electronic health record data sets is less common.

An In Vitro Delayed Scratch Closure Assay with Features of Chronic Wounds.

A scratch assay is a simple wound healing model for assessing cell migration in which a confluent cell layer is scratched to create a scratch zone ("wound") that cells migrate into. Here, we describe a scratch assay exhibiting delayed closure and increased inflammation, which more closely resemble chronic wounds. This delayed scratch closure assay is more relevant for screening compounds and studying delayed wound healing compared with traditional scratch wound assays that more closely resemble acute wounds.

Effect of 660-nm LED photobiomodulation on the proliferation and chondrogenesis of meniscus-derived stem cells (MeSCs).

Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm group were greater than those of the 4 J/cm and control groups.