[Antibodies to the structural and nonstructural gag-coded proteins of type-D retroviruses in patients with lymphadenopathy].

Screening of antibodies to structural and nonstructural gag gene-coded proteins in humans with lymphadenopathy and AIDS was performed by means of radioimmunoprecipitation (RIP) and western blotting. Pr78gag precursor of gag-coded proteins of type-D retrovirus from Hep-2 cells served as an antigen in RIP tests. Total number of sera (of humans with lymphoadenopathy) under RIP analysis was 18 and one sera of AIDS patient.

[Antibodies against structural proteins of carlaviruses in human and other mammals].

The study of four isolates of chrysanthemum B-virus (CVB) has shown the virus to have a single 40 Kd structural protein able to dissociate under the definite conditions forming the truncated (for 3 and 6 Kd) polypeptides having preserved their whole antigenic determinants. The human plasma is shown to contain antibodies reacting with the structural protein B-BX and, approximately 10 times weaker with the structural protein of another carlavirus, potato M-virus. Interaction of antibodies with CVB is found to take place due to F(ab)2 fragments.

[Antibodies to the structural proteins of the chrysanthemum B-virus in normal human plasma].

Electrophoretic analysis of the protein composition of chrysanthemum B virus (ChrBV), a member of the carlavirus family, performed in 7.5-15% gradient polyacrylamide gel with 0.1% SDS revealed the presence in the preparation of two proteins with molecular weights of 34,000 and 37,000.

[Advantages and disadvantages of silver staining of proteins in polyacrylamide gel].

Sensitivity of protein staining with Serva blue G-250 (Coomassie brilliant blue G-250 analogue) in polyacrylamide gel was determined. It has been shown that protein staining with 0.1% Serva blue G-250 results in the recovery of 80 to 35 ng of single protein, that is almost 10 times higher than reported previously for Coomassie brill.